Cynthia Hassinen

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A mathematical procedure was developed to predict the partition coefficients of the peptides AIIP, AWWP, AIIPAIIP and AWWPAWWP in poly(ethylene glycol) (PEG)/phosphate aqueous two-phase systems from amino acid hydrophobicities. In general, peptides containing tryptophan partition more into the PEG-enriched upper phase than analogous peptides containing(More)
Two different tetrapeptides, AlaTrpTrpPro and AlaIleIlePro, were inserted near the C-terminus of the protein ZZT0. The Trp-rich peptide unit strongly increased both the partitioning of ZZT0 into the polyethylene glycol (PEG)-rich phase in a PEG-potassium phosphate aqueous two-phase system and its retention on PEG and propyl hydrophobic interaction(More)
The two techniques aqueous two-phase partitioning and expanded bed adsorption that both are suitable for primary protein recovery were studied. Most of the work was focused on partition in aqueous two-phase systems and in particular on the possibility to effect the partition behaviour by fusion of short peptide tags or protein domains to the target protein.(More)
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