Cynthia D Wallace

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Methionine dependence is a metabolic defect found thus far only in transformed and malignant cells. The defect is manifested as the inability of cells to grow in media in which methionine (Met) is replaced by its immediate precursor homocysteine (Hcy). We have termed this Met- Hcy+ media. We demonstrate here that methionine-dependent cells derived from(More)
Methionine dependence is the inability of cells to grow when methionine (Met) is replaced by its immediate precursor homocysteine (Hcy) in the culture medium (Met-Hcy+ medium). All normal unestablished cell strains tested to date have been shown to be methionine-independent and thus grow almost as well in Met-Hcy+ medium as they do in Met+-Hcy-medium.(More)
We have isolated a variant of the MCF-7 human breast tumor that is characterized by a hormone-independent, yet hormone-responsive, phenotype. This tumor, designated MCF-WES, was derived from MCF-7 tumor cells implanted in the mammary fat pad of a nude mouse in the absence of estradiol supplementation. MCF-WES tumors remain responsive to estradiol; however,(More)
Many different types of cancer cells have been shown to be methionine-dependent. These cells, unlike normal cells, grow poorly or not at all when methionine is replaced by its immediate precursor homocysteine in the growth medium (Met- Hcy+ medium). We have previously shown that apparently normal total amounts of methionine are synthesized by(More)
Human prostate specimens commonly contain a spectrum of epithelial changes, including normal acinar and ductal structures, hyperplasia, intraepithelial neoplasia (dysplasia), and carcinoma. Since vascular endothelial growth factor (VEGF) expression is dependent on cell type and tissue microenvironment, meaningful quantitation of the levels of this mRNA in(More)
Primary synovial chondromatosis consists of cartilaginous metaplasia of the synovium, which leads to the production of intra-articular and periarticular osteocartilaginous bodies [8, 12, 18, 19]. The nomenclature has evolved with time to include chondromatosis of the joint capsule, diffuse enchondroma of the joint capsule, joint chondroma, synovial(More)
Immobilization-induced alterations occurred in young anterior cruciate ligament (ACL) samples, including the loss of the rounded appearance of the cells. The mature ACL was minimally altered by immobilization at the light microscopy level. In the immobilized young ACL the fibroblasts became elongated and there was loss of the normal pericellular matrix. The(More)
Basal skin resistance was measured continuously in sleeping human subjects. Instead of a hypothesized fall in basal skin resistance during periods of "dreaming" or emergent stage 1 electro-encephalographic activity, there was a rise which generally coincided with the "dreaming" period. This finding, along with other recent studies, indicates that this stage(More)
Site-specific methylation of the human c-myc oncogene was investigated in a set of cultured human tumor cell lines and normal human fibroblast strains. As previously reported, all of the tumor cell lines in contrast to normal cells were hypomethylated to various degrees in their total genomic DNA. The presence of methylation in specific regions of the c-myc(More)