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Ammopiptanthus mongolicus is the only evergreen broadleaf shrub in the northwest desert of China, which can survive long-term aridity and extremely cold environments. In order to understand the genetic mechanisms underlying stress tolerance and adaptation to unfavorable environments of woody plants, an EST approach was used to investigate expression(More)
Extracellular ATP (eATP) has been implicated in mediating plant growth and antioxidant defense; however, it is largely unknown whether eATP might mediate salinity tolerance. We used confocal microscopy, a non-invasive vibrating ion-selective microelectrode, and quantitative real time PCR analysis to evaluate the physiological significance of eATP in the(More)
Ammopiptanthus mongolicus, the only evergreen broadleaf shrub endemic to the northwest desert of China, is a valuable species for plant abiotic stress research. No report has so far described the selection of reference genes to get stringent normalization for qPCR in A. mongolicus. This work identified reliable reference genes for normalization of qPCR data(More)
Saussurea laniceps is a highly cold-tolerant, perennial, alpine herb that grows in Tibet, China. To reveal the reason why alpine plants can withstand large and frequent daily temperature fluctuation, we used S. laniceps callus to investigate the molecular mechanisms of freezing tolerance. The half lethal temperature (LT50) of S. laniceps calli decreased to(More)
Ammopiptanthus mongolicus is the only evergreen broadleaf shrub endemic to the Alashan Desert in northwestern China. The plants can survive temperatures of −30 °C or less in winter. A dehydrin-like protein gene, AmCIP, cloned from a cold-acclimated A. mongolicus seedling was transformed into E. coli. The transgenic strains exhibited enhanced freezing(More)
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