Cui-hua Liu

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Thermal stability of CO2 adducts of N-heterocyclic carbenes (NHCs) was studied by means of in situ FTIR method with monitoring of the nu(CO2) region of the infrared spectra under various conditions. 1,3-Bis(2,6-diisopropylphenyl)imidazolinium-2-carboxylate (SIPr-CO2) shows higher thermal stability compared with(More)
The ubiquitin-proteasome pathway (UPP) is the major system responsible for degradation of intracellular proteins in eukaryotes. By controlling the levels of key proteins, it regulates almost all of the cellular activities, including cell cycle progression, DNA replication and repair, transcription, protein quality control, immune response, and apoptosis.(More)
Studies on the whole herb of Urtica dioica L. grown in Nyingchi area, China's Tibet Autonomous Region, resulted in the isolated of nine compounds: beta-sitosterol, trans-ferulic acid, dotriacotane, erucic acid, ursolic acid, scopoletin, rutin, quercetin and p-hydroxylbenzalcohol. Dotriacotane, erucic acid, scopoletin, rutin and p-hydroxylbenzalcohol were(More)
To explore the application of DNA chip technology for the detection and typing of Human Papillomavirus (HPV), the HPV6, 11, 16 and 18 gene fragments were isolated and printed onto aminosilane-coated glass slides by a PixSys 5500 microarrayer as probes to prepare the HPV gene chips. HPV samples, after being labeled with fluorescent dye by restriction display(More)
The honeybee product propolis and its extracts are known to have biological effects such as antibiotic, anti-viral, anti-inflammatory and anti-tumor activities. This study was designed to investigate whether water-soluble propolis (WSP) inhibits tumor growth. The tumor cell line used was mouse sarcoma 180 (S-180), and its growth was determined in vitro and(More)
OBJECTIVE To study the application of Agilent 2100 Bioanalyzer in the study of gene differential expression. METHODS The total RNAs were extracted and purified from Saccharomyces cerevisiae to synthesize double-stranded cDNAs by reverse transcription. Restriction display-PCR was employed to obtain the cDNA fragments, which were examined by Agilent 2100(More)
OBJECTIVE To detect the possible presence of AI-2 quorum-sensing pathway and construct group B Streptococcus (GBS) mutants with deletion of LuxS gene related to quorum-sensing pathway. METHOD V. harveyi BB170 was employed as the reporter strain to detect AI-2 pathway in GBS, and identification of LuxS homologous gene in GBS type V strain 2603 was(More)
OBJECTIVE To explore the feasibility of using Agilent 2100 bioanalyzer in the detection and genotyping of human papilloma virus (HPV). METHODS The consensus sequence of highly conserved region (L1) and genotype-specific gene fragments of all HPV genotypes (including HPV6, 11, 16 and 18) were amplified by PCR technique using general and type-specific(More)
OBJECTIVE To explore the mechanism of virulence regulation in group B streptococcus (BGS) by studying LuxS-related AI-2 quorum-sensing pathway in GBS. METHODS luxS gene deletion mutants of GBS (Delta lusX) were characterized by reverse transcription (RT)-PCR, colony immunoblot analysis, growth curve measurement, and cAMP determination. Functional analysis(More)
This study explores the restriction display-polymerase chain reaction (RD-PCR) application of a new chip-based nucleic acid analysis system (Agilent 2100 bioanalyser) in a gene differential expression study. Total RNAs is extracted from Saccharomyces cerevisiae, double-stranded complementary DNA (cDNA) is synthesised by reverse transcription from the(More)