Cristina Di Poto

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MOTIVATION Liquid chromatography-mass spectrometry (LC-MS) has been widely used for profiling expression levels of biomolecules in various '-omic' studies including proteomics, metabolomics and glycomics. Appropriate LC-MS data preprocessing steps are needed to detect true differences between biological groups. Retention time (RT) alignment, which is(More)
Defining clinically relevant biomarkers for early stage hepatocellular carcinoma (HCC) in a high-risk population of cirrhotic patients has potentially far-reaching implications for disease management and patient health. Changes in glycan levels have been associated with the onset of numerous diseases including cancer. In the present study, we used liquid(More)
—In quantitative liquid chromatography-mass spec-trometry (LC-MS) experiments, variability assessment helps improve experimental design and detect true differences in ion abundance. A peak-level mixed effects model is considered to estimate the variability due to heterogeneity of the biological samples, inconsistency in sample preparation, and instrument(More)
A fundamental challenge in quantitation of biomolecules for cancer biomarker discovery is owing to the heterogeneous nature of human biospecimens. Although this issue has been a subject of discussion in cancer genomic studies, it has not yet been rigorously investigated in mass spectrometry based proteomic and metabolomic studies. Purification of mass(More)
Gas chromatography coupled with mass spectrometry (GC-MS) is one of the technologies widely used for qualitative and quantitative analysis of small molecules. In particular, GC coupled to single quadrupole MS can be utilized for targeted analysis by selected ion monitoring (SIM). However, to our knowledge, there are no software tools specifically designed(More)
This study evaluates changes in metabolite levels in hepatocellular carcinoma (HCC) cases vs. patients with liver cirrhosis by analysis of human blood plasma using gas chromatography coupled with mass spectrometry (GC-MS). Untargeted metabolomic analysis of plasma samples from participants recruited in Egypt was performed using two GC-MS platforms: a GC(More)
  • Rania Esteitie, Oluwatoyin Adewuyi, Murugan Ravindran, Yin Zheng, Samantha Merck, Christian Timbol +3 others
  • 2012
We also found a decrease in FEV1 comparing the 3 groups with a P , .011 morbid versus eutrophic and P , .049 morbid versus obese. Conclusions: Our results confirm the findings of others, who have shown that lung volumes especially FRC and ERV decrease as body weight increases. Obese patients have a combination of mechanical and inflammatory effects that(More)
Background: Medical questionnaires are important for assessing allergy patients. However, current methods of administering questionnaires are cumbersome, expensive, and laborious to accurately administer, track and score. eZhengtricity is a simple Google Documents based workflow, coupled with customized Excel formulas, that allow researchers to create,(More)
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