Cristina Boero

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— Nano-biosensing provides new tools to investigate cellular differentiation and proliferation. Upon the various metabolic compounds secreted by cells during life cycles, glucose, lactate and hydrogen peroxide (H2O2) are of first interest. Nano-structured electrodes may enhance the compounds sensitivity in order to precisely detect cell cycle variation. In(More)
Monitoring of metabolic compounds in cell cultures can provide real-time information of cell line status. This is particularly important in those lines not fully known, as the case of embryonic and mesenchymal cells. On the other hand, such approach can pave the way to fully automated systems for growing cell cultures, when integrated in Petri dishes. To(More)
An investigation on nano-structured electrodes to detect different metabolites is proposed in this paper. Three different metabolites are considered: glucose, lactate, and cholesterol. The direct detection of hydrogen perox-ide is also considered since it does not involve any enzyme. The metabolites and the peroxide were detected by using screen-printed(More)
The objective of this work is the systematic study of the use of electrochemical readout for advanced diagnosis and drug monitoring. Whereas to date various electrochemical principles have been studied and successfully tested, they typically operate on a single target molecule and are not integrated in a full data analysis chain. The present work aims to(More)
— A novel single-chip and multiplexed read-out circuit for multi-electrode electrochemical sensors, in standard 0.18 μm UMC CMOS technology, is presented. The circuit is a part of a fully-integrated biochip (in design) for the detection of multiple metabolites. The proposed topology is based on the potentiostat approach, and it is devoted to detect currents(More)
Carbon nanotubes have been attracting a lot of interest as electron transfer mediators to enhance electrochemical biosensing. The main reason behind this is usually recognized in terms of augmented electrochemical active surface area. The aim of this paper is to review other phenomena that occur at the electrochemical interface. Three distinct features of(More)
—The IronIC project has the aim of developing a fully implantable and remotely powered platform for the real-time monitoring of human metabolites. In this paper we present a mixed-signal interface IC for the electrochemical sensing data acquisition chain. The IC controls and reads out up to five biomolecular sensors, by receiving commands from a standard(More)
— A novel system for remote monitoring of metabolism in an animal model is proposed in this paper. The system is obtained by integrating bio-nano-sensors to detect single-metabolites, an electrochemical front-end made with off-the-shelf components, a radio frequency communication subsystem , and an antenna of new design. The system has been calibrated and(More)
Amperometric biosensors are complex systems and they require a combination of technologies for their development. The aim of the present work is to propose a new approach in order to develop nanostructured biosensors for the real-time detection of multiple metabolites in cell culture flasks. The fabrication of five Au working electrodes onto silicon(More)
Conventional pharmaceutical processes involving cell culture growth are generally taken under control with expensive and long laboratory tests performed by direct sampling to evaluate quality. This traditional and well-established approach is just partially adequate in providing information about cell state. Electrochemical enzyme-based biosensors offer(More)