Craig S Brinkworth

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Ricin is a toxic protein produced in the seeds of the castor bean plant. The toxicity of the protein and the ease in which it can be extracted from the seeds makes it a potential biological warfare agent. There has been extensive work in the development of analytical techniques that can identify the protein robustly and rapidly. On-target tryptic digestion(More)
The recent development of novel fragmentation processes based on either electron capture directly or transfer from an anion show great potential for solving problems in proteomics that are intractable by the more widely employed thermal-based fragmentation processes such as collision induced dissociation. The dominant fragmentation occurring upon electron(More)
Ricin is a highly toxic protein from the seeds of the castor bean plant. Crude extracts from castor beans are toxic by several routes, and there is international concern about the use of these extracts by terrorist organizations. Lethality in aerosolized form has spurred the development of methods for the rapid detection of this protein from air samples(More)
We present results of high time resolution photometry of the eclipsing pre-cataclysmic variable NN Ser. NN Ser is a white dwarf/M dwarf binary with a very low-mass secondary star (∼ 0.2 M ⊙). We observed 13 primary eclipses of NN Ser using the high-speed CCD camera ULTRACAM and derived times of mid-eclipse, from fitting of light curve models, with(More)
Buffers and detergents are notorious for suppression of analyte signal in electrospray and MALDI mass spectrometry and, invariably, analysts will take steps to remove these contaminants before MS analysis. However, we have found serendipitously that protein signal with MALDI MS is improved by about an order of magnitude on the addition of small amounts of(More)
Recently, we showed that the signal intensity of intact protein by matrix-assisted laser desorption/ionisation (MALDI) mass spectro-metry measurement can be enhanced at least an order of magnitude by the addition of Tween80 to the analyte solution. We did not ascertain whether this effect was limited to Tween80 or if it was more universal of biological(More)
We present high-speed, three-colour photometry of the faint eclipsing cataclysmic variables XZ Eri and DV UMa. We determine the system parameters through two techniques: first, timings of the eclipse contact phases of the white dwarf and bright-spot using the derivative of the light curve; and secondly, a parameterized model of the eclipse fitted to the(More)
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