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Whole-cell patch-clamp electrophysiology of neurons is a gold-standard technique for high-fidelity analysis of the biophysical mechanisms of neural computation and pathology, but it requires great skill to perform. We have developed a robot that automatically performs patch clamping in vivo, algorithmically detecting cells by analyzing the temporal sequence(More)
The surface topography of thin, transparent materials is of interest in many areas. Some examples include glass substrates for computer hard disks, photomasks in the semiconductor industry, flat panel displays, and x-ray telescope optics. Some of these applications require individual foils to be manufactured with figure errors that are a small fraction of a(More)
The Reflection Grating Spectrometer (RGS) on Constellation-X will require thousands of large gratings with very exacting tolerances. Two types of grating geometries have been proposed. In-plane gratings have low ruling densities (∼ 500 l/mm) and very tight flatness and assembly tolerances. Off-plane gratings require much higher ruling densities (∼ 5000(More)
The success of using thin substrates in various fields has urged researchers to further study the possibilities of improving the technology for future applications. For example, the high surface-area-to-weight ratio and strength of sheet glass allow flat-panel display technology to result in high-definition televisions that can be hung on walls like(More)
The proposed Reflection Grating Spectrometer (RGS) on the Constellation–X mission is designed to provide high-resolution x-ray spectroscopy of astrophysical sources. Two types of reflection grating geometries have been proposed for the RGS. In-plane gratings have relatively low-density rulings (∼ 500 lines/mm) with lines perpendicular to the plane of(More)
The mainstay of treatment for thrombosis, the formation of occlusive platelet aggregates that often lead to heart attack and stroke, is antiplatelet therapy. Antiplatelet therapy dosing and resistance are poorly understood, leading to potential incorrect and ineffective dosing. Shear rate is also suspected to play a major role in thrombosis, but(More)
Silicon microcombs developed at our laboratory for the precision alignment and assembly of large-area foil optics have previously been demonstrated to achieve submicron-level assembly repeatability with submillimeter-thick flat substrates. In this article we report on a double-side deep reactive-ion etch fabrication process using silicon-on-insulator wafers(More)
The Reflection Grating Spectrometer (RGS) on Constellation-X is designed to supply astronomers with high spectral resolution in the soft x-ray band from 0.25 to 2 keV. High resolution, large collecting area and low mass at grazing incidence require very flat and thin grating substrates, or thin-foil optics. Thin foils typically have a diameter-to-thickness(More)
BACKGROUND Prior exposure to stress is a risk factor for developing posttraumatic stress disorder (PTSD) in response to trauma, yet the mechanisms by which this occurs are unclear. Using a rodent model of stress-based susceptibility to PTSD, we investigated the role of serotonin in this phenomenon. METHODS Adult mice were exposed to repeated(More)
Pathologic changes in intracranial pressure (ICP) are commonly observed in a variety of medical conditions, including traumatic brain injury, stroke, brain tumors, and glaucoma. However, current ICP measurement techniques are invasive, requiring a lumbar puncture or surgical insertion of a cannula into the cerebrospinal fluid (CSF)-filled ventricles of the(More)