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The breadth of genomic diversity found among organisms in nature allows populations to adapt to diverse environments. However, genomic diversity is difficult to generate in the laboratory and new phenotypes do not easily arise on practical timescales. Although in vitro and directed evolution methods have created genetic variants with usefully altered(More)
Optogenetic inhibition of the electrical activity of neurons enables the causal assessment of their contributions to brain functions. Red light penetrates deeper into tissue than other visible wavelengths. We present a red-shifted cruxhalorhodopsin, Jaws, derived from Haloarcula (Halobacterium) salinarum (strain Shark) and engineered to result in red(More)
Whole-cell patch-clamp electrophysiology of neurons is a gold-standard technique for high-fidelity analysis of the biophysical mechanisms of neural computation and pathology, but it requires great skill to perform. We have developed a robot that automatically performs patch clamping in vivo, algorithmically detecting cells by analyzing the temporal sequence(More)
We present nanoNS3, a network simulator for modeling Bacterial Molecular Communication (BMC) networks. nanoNS3 is built atop the Network Simulator 3 (ns-3). nanoNS3 is designed to achieve the following goals: 1) accurately and realistically model real world BMC, 2) maintain high computational efficiency, 3) allow newly designed protocols to be implemented(More)
The surface topography of thin, transparent materials is of interest in many areas. Some examples include glass substrates for computer hard disks, photomasks in the semiconductor industry, flat panel displays, and x-ray telescope optics. Some of these applications require individual foils to be manufactured with figure errors that are a small fraction of a(More)
Robotic and automation technologies have played a huge role in in vitro biological science, having proved critical for scientific endeavors such as genome sequencing and high-throughput screening. Robotic and automation strategies are beginning to play a greater role in in vivo and in situ sciences, especially when it comes to the difficult in vivo(More)
The Reflection Grating Spectrometer (RGS) on Constellation-X will require thousands of large gratings with very exacting tolerances. Two types of grating geometries have been proposed. In-plane gratings have low ruling densities (∼ 500 l/mm) and very tight flatness and assembly tolerances. Off-plane gratings require much higher ruling densities (∼ 5000(More)
The mainstay of treatment for thrombosis, the formation of occlusive platelet aggregates that often lead to heart attack and stroke, is antiplatelet therapy. Antiplatelet therapy dosing and resistance are poorly understood, leading to potential incorrect and ineffective dosing. Shear rate is also suspected to play a major role in thrombosis, but(More)