Learn More
The ob gene product, leptin, is an important circulating signal for the regulation of body weight. To identify high affinity leptin-binding sites, we generated a series of leptin-alkaline phosphatase (AP) fusion proteins as well as [125I]leptin. After a binding survey of cell lines and tissues, we identified leptin-binding sites in the mouse choroid plexus.(More)
PURPOSE The aim of this study was to validate quantitative metabolic response of tumors to a treatment measured by longitudinal 2-deoxy-2-[(18)F]fluoro-D-glucose (FDG) micro positron emission tomography (microPET) as a robust tool for preclinical evaluation of new anticancer agents. PROCEDURES Severe combined immunodeficiency mice with CWR22 xenografts(More)
UNLABELLED BACKGROUND Arterial thrombosis triggered by vascular injury is a balance between thrombus growth and thrombus fragmentation (dethrombosis). Unbalance towards thrombus growth can lead to vascular occlusion, downstream ischemia and tissue damage.Here we describe the development of a simple methodology that allows for continuous real time(More)
To facilitate drug discovery directed toward platelet-specific targets, we developed a platelet isolation and fluorophore-loading method that yields functionally responsive platelets in which we were able to detect agonist-induced calcium flux using a microfluidics-based screening platform. The platelet preparation protocol was designed to minimize(More)
SUMMARY Measurements of the sodium content of single oocyte nuclei of Triturus cristatus carnifex at various stages of maturation, show a rise in concentration in eggs of the size 0-5-0-6 mm diameter. When the nuclei are isolated rapidly under paraffin, the absolute values obtained for the concentration are much lower than have been previously reported for(More)
  • 1