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With the goal of identifying key factors involved in human breast cancer progression, we applied human cytokine antibody arrays we have developed to screen cytokine expression levels in human breast cancer cell lines and identified interleukin (IL)-8 as a key factor involved in breast cancer invasion and angiogenesis. Elevated expression of IL-8 in breast(More)
Modern methods of microarray data analysis are biased towards selecting those genes that display the most pronounced differential expression. The magnitude of differential expression does not necessarily indicate biological significance and other criteria are needed to supplement the information on differential expression. Three large sets of microarray(More)
In searching for small-molecule compounds that inhibit proliferation and survival of diffuse large B-cell lymphoma (DLBCL) cells and may, therefore, be exploited as potential therapeutic agents for this disease, we identified the commonly used and well-tolerated antibiotic doxycycline as a strong candidate. Here, we demonstrate that doxycycline inhibits the(More)
Computer security has become vital for protecting users, applications and data, yet the field still faces severe shortages in skilled professionals. Typical methods to teach security from textbooks and academic papers are not engaging and take considerable time. Our hypothesis is that a security game that closely emulates real-world systems can improve(More)
The expression and stability of the estrogen receptor (ER) is the result of a complex process that is modulated by estrogens and antiestrogens. Regulation of the steady-state ER mRNA and protein levels in breast cancer cells appears to be the result of either of two distinct regulatory mechanisms. Estrogen exposure causes a rapid down-regulation of the(More)
This investigation examines the tamoxifen (TAM)-dependent growth in vivo of an MCF-7 tumor variant, MCF-7TAM, previously reported in Ovariectomized athymic mice were implanted with I-mm-' pieces of MCF-7TAM and were treated with Silastic capsules of varying sizes containing TAM to demonstrate dose-dependent growth over a 10-wk experiment. TAM was necessary(More)
Long-term tamoxifen (TAM) therapy was examined in athymic mice bearing MCF-7 tumors of different sizes. Six months of TAM treatment caused no increase in tumor size (compared to placebo treatment) for animals treated initially following implantation of tumor pieces (approx imately 1 mm3) or for animals with 0.2-cm2 tumors (grown with 1 month of estrogen(More)
The crystallization of the ligand-binding domain (LBD) of the estrogen receptor (ER) with 17ß-estradiol and raloxifene [A. M. Brzozowski et al., Nature (Lond.), 389: 753†" 758,1997] now provides a molecular basis for the biological activity of complexes as either agonists or antagonists. It is well established that the critical structural feature of(More)
These studies have evaluated the modulation by epidermal growth factor (EGF) of estrogen receptor (ER) levels and estradiol-stimulated progesterone receptor (PgR) synthesis. Short-term culture of MCF-7 cells in an "estrogen (phenol red indicator)-free" environment caused a rise in ER concentration that is inhibited by EGF at IO"8 M and 10~7M. Estradici at(More)