Craig E. Forney

Learn More
We have investigated the use of charged fusion tails with the enzyme glucoamylase in reversed micellar extraction. The addition of the charged tails increased the fraction of enzymatically active protein recovered at a given pH, with the tails containing the largest number of charges being recovered at the highest level. The series of mutations also allows(More)
Behavior of a series of fusion proteins of varying charge in reversed micellar extraction was studied. The proteins consisted of the enzyme glucoamylase from Aspergillus awamori joined to short peptides containing from 0-10 additional aspartate residues. The fusions were partitioned into two different cationic surfactant systems, one based on the surfactant(More)
  • 1