Craig Duckham

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Yeast cells can be used as biocompatible and biodegradable containers for the microencapsulation of a variety of actives. Despite the wide application of this process, e.g. in the food industry, mechanism and controlling factors are yet poorly known. In this study we have studied kinetics and mechanistic aspects of the spontaneous internalization of(More)
The aim of this study was to investigate the effect of heat-killed yeast cells on the integrity of epithelial tight junctions in vitro. Changes in barrier potential of Caco-2 cell monolayers were assessed by transepithelial electrical resistance (TEER) measurements and by an increasing permeability to a marker protein, horse–radish peroxidase (HRP).(More)
We here report a study on the passive permeability of hydrophobic probes through the cell wall of Saccharomyces cerevisiae. In this study we have prepared a series of fluorescent probes with similar chemical composition and molecular weight ranging from a few hundreds to a few thousands of g mol(-1). Their permeation into the cell body exhibits a clear MW(More)
conditions is not new; history tells us of the many benefits of microbes to man. Their potential in the prevention and treatment of a wide range of disease conditions has been recognised since the days of Jenner, who in 1796 inoculated a young boy with cowpox and later with smallpox. The use of modified non-virulent species, attenuated bacteria/viruses and(More)
We here report on a preparative, template-based method for oxidation-responsive, poly(propylene sulfide) cross-linked nanoparticles. In this study we demonstrate that preformed, narrow polydispersity, and end-functional polysulfides can be dispersed to yield stable emulsions, which can then be converted into stable nanoparticles through photochemically(More)
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