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OBJECTIVE To evaluate endometrial adhesion and invasion of peritoneal mesothelium. DESIGN Descriptive study using confocal laser-scanning microscopy. SETTING University-based laboratory. PATIENT(S) Women undergoing surgery for benign conditions. INTERVENTION(S) Fluorescence-labeled peritoneal mesothelial cells (PMCs) were grown on coverslips.(More)
OBJECTIVE To characterize the source of variability in endometrial stromal cell (ESC) binding to peritoneal mesothelial cells (PMC). DESIGN In vitro study. SETTING University medical center. PATIENT(S) Reproductive-age women without endometriosis undergoing surgery for benign conditions. INTERVENTION(S) None. MAIN OUTCOME MEASURE(S) Binding of(More)
OBJECTIVE To evaluate the initial adhesion of endometrium to the peritoneum. DESIGN Descriptive study using light and confocal laser-scanning microscopy, immunohistochemistry, and transmission electron microscopy. SETTING University-based laboratory. PATIENT(S) Women without endometriosis undergoing surgery for benign conditions. INTERVENTION(S)(More)
Trophectoderm (TE) biopsy and DNA microarray have become the new technologies for preimplantation genetic diagnosis in humans. In this study, we comprehensively examined aneuploid formation in human blastocysts produced in vitro with microarray and investigated the clinical outcome after transfer of euploid embryos. Biopsied cells from either TE or inner(More)
OBJECTIVE To localize the extracellular matrix proteins collagen I, collagen IV, fibronectin, and laminin in the peritoneal membrane. STUDY DESIGN Peritoneal biopsies (n = 13) from the anterior abdominal wall and the uterine serosa (n = 3) were incubated with antibodies to collagen IV, laminin, collagen I, and fibronectin. Specimens were examined using(More)
OBJECTIVE To characterize the expression of alpha subunits of integrin adhesion molecules in peritoneal tissue in vivo and in vitro. METHOD Peritoneum from the anterior abdominal wall (n = 22) and the serosa of the posterior uterus (n = 11) was obtained from women of reproductive age without endometriosis who were undergoing surgery for benign conditions.(More)
BACKGROUND High proportions of human embryos produced by in vitro fertilization are aneuploidy and mosaic. DNA microarray is one of the most practical screening methods to select euploid embryos for transfer. However, mosaic pregnancy is still possible due to embryonic mosacism. Here we report a successful pregnancy after transfer of a mosaic blastocyst(More)
The aim of this study was to investigate whether activin A has an effect on the attachment and/or invasion of endometrial cells in a modeled peritoneum in vitro. Cultured endometrial stromal cells (ESCs) and endometrial epithelial cells (EECs) were treated with activin A (6.25-50 ng/ml) and with activin A (25 ng/ml) with and without inhibin A or(More)
OBJECTIVE To determine the role of peritoneal mesothelial cells (PMCs) in the process of endometrial invasion into the peritoneum and to evaluate gene expression after endometrial-PMC co-culture. DESIGN In vitro study. SETTING University laboratory. PATIENT(S) Reproductive-age women without endometriosis. INTERVENTION(S) None. MAIN OUTCOME(More)
OBJECTIVE To define the risk of human cytomegalovirus (HCMV) transmission from donated oocytes. DESIGN Prospective study. SETTING University IVF program. PATIENT(S) Sixty-seven couples undergoing 72 cycles of IVF-ET. INTERVENTION(S) None. MAIN OUTCOME MEASURE(S) Serum from both partners (women: n = 71; men: n = 60) was obtained for detection of(More)