Corzo Gerardo

Learn More
The gene of the four disulfide-bridged toxin II was cloned into the expression vector pQE30 Centruroides suffusus suffusus containing a 6His-tag and an FXa proteolytic cleavage region. This recombinant vector was transfected into BL21 cells and E. coli expressed under induction with isopropyl thiogalactoside (IPTG). The level of expression was 24.6 mg/L of(More)
The current nursing shortage problem as defined in this article, is the result of a supply and demand imbalance. The demand for nurses is growing at a faster rate than the supply. If strategies are not implemented to correct this imbalance the demand for nurses will continue to surpass the supply, negatively affecting access to and delivery of quality(More)
  • 1