Constantine P. Karakousis

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Cytogenetic analysis of short-term cultures was carried out on 109 lipomas from 92 patients. Clonal chromosomal abnormalities were present in 50% of the tumors analyzed. Based on the results, three main cytogenetic groups were identified and included: (a) tumors with normal karyotypes, (b) tumors with abnormalities involving region q13-15 on chromosome 12,(More)
BACKGROUND Sentinel-node biopsy, a minimally invasive procedure for regional melanoma staging, was evaluated in a phase 3 trial. METHODS We evaluated outcomes in 2001 patients with primary cutaneous melanomas randomly assigned to undergo wide excision and nodal observation, with lymphadenectomy for nodal relapse (observation group), or wide excision and(More)
The insulin-like growth factors I and II (IGF-I and -II) are proteins which stimulate cell proliferation and are important in normal human growth and development. They are coded for by separate genes and bind to specific cell surface receptors, eliciting a mitogenic response. IGFs are secreted by several cell lines derived from adult tumors. We have(More)
Colony growth in soft agar of cells disaggregated from 87 solid tumor specimens was evaluated. Tumors were disaggre gated by an enzymatic method consisting of microtome slicing of tissues and incubation at 37° for 2 hr in Roswell Park Memorial Institute Tissue Culture Medium 1640 containing 10% fetal bovine serum, 0.8% collagenase II, and 0.002% DNase I.(More)
Characterization of cells comprising solid tumors will facilitate the rational design of cancer chemotherapy for individual patients. We have prepared cell suspensions from human melanoma, sarcoma, and lung tumors by thinly slicing the tissue with a microtome and scalpels (mechanical release), followed by treatment with a mixture of collagenase II and DNase(More)
BACKGROUND The aim of this study is to evaluate the prognostic parameters and treatment modalities of malignant melanoma patients with brain metastases. METHODS EXPERIMENTAL DESIGN a retrospective study with a mean follow-up of 46 months. SETTING specialized Cancer Center. PATIENTS the charts of 136 patients, treated in Roswell Park Cancer(More)
Colony growth in soft agar of cells disaggregated from 87 solid tumor specimens was evaluated. Tumors were disaggre gated by an enzymatic method consisting of microtome slicing of tissues and incubation at 37° for 2 hr in Roswell Park Memorial Institute Tissue Culture Medium 1640 containing 10% fetal bovine serum, 0.8% collagenase II, and 0.002% DNase I.(More)
High sacral resections may best be performed with the use of fine rongeurs to divide the fused sacral laminae at the proposed line of transection of the bone posteriorly. Thus, the sacral canal is opened, and with gentle dissection, the dural sac and sacral roots at this level are displaced superiorly and laterally. At this level, the fused sacral bodies(More)
Colony growth in soft agar of cells disaggregated from 87 solid tumor specimens was evaluated. Tumors were disaggre gated by an enzymatic method consisting of microtome slicing of tissues and incubation at 37° for 2 hr in Roswell Park Memorial Institute Tissue Culture Medium 1640 containing 10% fetal bovine serum, 0.8% collagenase II, and 0.002% DNase I.(More)
The present issue of the International Journal of Surgical Oncology on Pelvic Surgery contains a series of articles on prostate cancer, gynecologic malignancies, and rectal cancer. The article on " radical prostatectomy as a first-line treatment in patients with initial PSA >20 ng/mL " by Hinev et al. reports on patients diagnosed with prostate cancer (PCa)(More)