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Biosynthesis of chiral 3-hydroxyvalerate from single propionate-unrelated carbon sources in metabolically engineered E. coli
- Hsien-Chung Tseng, Catey Harwell, Collin H. Martin, K. Prather
- BiologyMicrobial cell factories
- 1 November 2010
This work represents the first report of direct microbial production of enantiomerically pure 3HV from a single carbon source using synthetic metabolic pathways in recombinant strains of Escherichia coli.
A platform pathway for production of 3-hydroxyacids provides a biosynthetic route to 3-hydroxy-γ-butyrolactone.
- Collin H. Martin, H. Dhamankar, Hsien-Chung Tseng, Micah J. Sheppard, C. Reisch, K. Prather
- BiologyNature communications
- 29 January 2013
A novel pathway to produce various chiral 3-hydroxyacids, including 3,4-dihydroxybutyric acid, consisting of enzymes that condense two acyl-CoAs, stereospecifically reduce the resulting β-ketone and hydrolyze the CoA thioester to release the free acid is designed.
De novo biosynthetic pathways: rational design of microbial chemical factories.
Metabolic Engineering of Escherichia coli for Enhanced Production of (R)- and (S)-3-Hydroxybutyrate
- Hsien-Chung Tseng, Collin H. Martin, D. Nielsen, K. Prather
- Biology, EngineeringApplied and Environmental Microbiology
- 20 March 2009
This study reports the highest (S)-3HB titer achieved in shake flask E. coli cultures to date, and profiled three thiolase homologs (BktB, Thl, and PhaA) and two coenzyme A (CoA) removal mechanisms to promote maximal activity.
Engineering E. coli for the biosynthesis of 3-hydroxy-γ-butyrolactone (3HBL) and 3,4-dihydroxybutyric acid (3,4-DHBA) as value-added chemicals from glucose as a sole carbon source.
High-titer production of monomeric hydroxyvalerates from levulinic acid in Pseudomonas putida.
Synthetic metabolism: engineering biology at the protein and pathway scales.
Improved synthesis of proline-derived Ni(II) complexes of glycine: versatile chiral equivalents of nucleophilic glycine for general asymmetric synthesis of alpha-amino acids.
- Hisanori Ueki, Trevor K. Ellis, Collin H. Martin, Tomas U Boettiger, Shawna B. Bolene, V. Soloshonok
- ChemistryThe Journal of organic chemistry
- 6 August 2003
The key step of the method is the synthetically efficient amid bond formation between the corresponding o-aminobenzophenones, featuring significant steric shielding and low nucleophilicity of the amino functionality as well as sterically constrained (S)-N-benzylproline (BP).
Efficient Large‐Scale Synthesis of Picolinic Acid‐Derived Nickel(II) Complexes of Glycine
An efficient, large-scale method for the preparation of 2-[N-(α-picolyl)amino]acetophenone (PAAP) and 2-[N-(α-picolyl)amino]benzophenone (PABP), the hitherto unknown…
Efficient synthesis of serically constrained smmetrically alpha,alpha-disubstituted alpha-amino acids under operationally convenient conditions.
- Trevor K. Ellis, Collin H. Martin, Gary M. Tsai, Hisanori Ueki, V. Soloshonok
- Chemistry, BiologyThe Journal of organic chemistry
- 4 July 2003
High chemical yields of the dialkylated products, combined with the simplicity of the experimental procedure, render this method worth immediate use for multigram scale preparation of the sym-alpha,alpha-AA.