Colleen Trombley

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We investigated the epidemiology and clinical features of invasive S. pyogenes infection in a pediatric population over a 7-year period (1984-90) by retrospective review. An increasing frequency in invasive infections had occurred (0-11.81/10,000 admissions). A large proportion (48%) of these were orthopedic infections. An epidemic strain was typed as M1T1.(More)
Candida dubliniensis is a newly recognized species of yeast, which may have been forrmerly identified as Candida albicans, that has been rarely isolated from invasive fungal infections among humans. The authors document a C. dubliniensis fungemia that occurred during the course of a vascular access infection in a 2-year-old who was undergoing active therapy(More)
CONTEXT Meningococcal infection has a high public profile because of its dramatic presentation, high fatality rate, and propensity to occur in outbreaks and clusters of cases. Use of a diagnostic polymerase chain reaction (PCR) assay could enhance laboratory confirmation of cases and guide the public health response in North America. OBJECTIVE To assess(More)
A rapid diagnostic procedure, which is based upon the polymerase chain reaction (PCR) genetic amplification technology, was utilized to establish the presence of Bordetella pertussis in nasopharyngeal washes from children. Overall, 14.7% of 456 specimens were positive by either culture or the rapid assay. Culture and PCR were concordant for 62.7% of(More)
OBJECTIVE Define the applicability of a rapid molecular typing scheme to study the epidemiology of a Serratia marcescens outbreak. DESIGN With the assistance of a simple bacterial lysis technique, isolates of S marcescens from a putative outbreak were genotyped with the polymerase chain reaction technology for which primers were chosen on the basis of(More)
One hundred and six specimens from 90 patients with cystic fibrosis were evaluated for the presence of Burkholderia cepacia using a current routine diagnostic protocol as well as a research protocol involving polymyxin B-MacConkey agar without crystal violet, PC agar, OFPVL agar, and a selective brain-heart infusion broth. Ten specimens from eight patients(More)
In the SIGNAL (Oxoid Ltd., Basingstoke, United Kingdom) blood culture system, gas produced by bacterial metabolism displaces medium from the culture bottle into an upper reservoir via a hollow needle. Displacement of media may provide a visual indication of the presence of both aerobic and anaerobic organisms in a single medium. The single-bottle SIGNAL(More)
Insertion sequence primers originally intended to amplify a singular specific product for the rapid diagnosis of Bordetella pertussis respiratory infection were used to differentiate strains of Pseudomonas (Burkholderia) cepacia. A modified sample preparation of proteinase K treatment and boiling was used in lieu of DNA extraction. The method was simple,(More)
The sensitivity of the BACTEC NR660 blood culture system was assessed by using paired bottles of anaerobic (NR7A) and resin-containing aerobic (NR16A) media and conditions and organisms which simulated those found in pediatric practice. Corresponding media (7D and 16B) of the established BACTEC 460 radiometric method served as controls. The performances of(More)