Learn More
In cell-free extracts derived from Xenopus eggs which oscillate between S phase and mitosis, incompletely replicated DNA blocks the activation of p34cdc2-cyclin by maintaining p34cdc2 in a tyrosine-phosphorylated form. We used a recombinant cyclin fusion protein to generate a substrate to measure the ability of the tyrosine kinase(s) to phosphorylate and(More)
Dissociation and association of membranes with chromatin at the beginning and end of mitosis are critical in controlling nuclear dynamics during these stages of the cell cycle. Employing purified membrane and cytosolic fractions from Xenopus eggs, a simple assay was developed for the reversible binding of nuclear membrane vesicles to chromatin. We have(More)
In this paper we analyse the performance of Mobile IP (MIP) handoffs with respect to the three available MIP movement detection methods, namely Lazy Cell Switching, Prefix Matching and Eager Cell Switching. The network scenario considered involves single-agent subnetworks and Mobile Nodes that do not have the capability to contemporarily participate in(More)
Recipients of this document are requested to submit, with their comments, notification of any relevant patent claims or other intellectual property rights of which they may be aware that might be infringed by any implementation of the specification set forth in this document, and to provide supporting documentation. IMS takes no position regarding the(More)
This paper presents a survey of recent approaches in the application of the object-oriented paradigm to formal specification. The complexity of current information systems demands the use of a higher degree of formalism in the development process. Formal languages such as Z, VDM and Lotos have been used extensively in academic environments and research(More)
The latest paradigm for the development of distributed systems is the Service-oriented Architecture (SoA) with implementation based upon Web Services. In principle, the development of a distributed system using a model driven approach enables the automated implementation and testing of the system from the specification of the component services. An(More)