Colin Griffith

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The H(+)-ATPase of tonoplast vesicles isolated from red beet (Beta vulgaris L.) storage tissue was studied with respect to the kinetic effects of Cl(-) and NO(3) (-). N-Ethylmaleimide (NEM) was employed as a probe to investigate substrate binding and gross conformational changes of the enzyme. Chloride decreased the K(m) of the enzyme for ATP but caused(More)
Here, a Web of Things case study for agriculture focuses on an experimental smart farm that uses a range of environmental sensors and livestock monitoring technologies. An ontology-enabled architecture that permits personal alert specification was applied on the farm and the results analyzed; the observations are published as a linked data cube, which(More)
AIM The aim of this paper was to discuss the predictive validity and reliability of the Community Client Need Classification System (CCNCS), the results of using this tool and public health nurses' satisfaction with the system. BACKGROUND Public health nurses provide the majority of community nursing services in Ireland. The traditional method of(More)
The H+-ATPase of Beta vacuolar membrane (tonoplast) comprises at least three functionally distinct subunits of Mr = 67,000, 57,000, and 16,000, respectively (Manolson, M. F., Rea, P. A., and Poole, R. J. (1985) J. Biol. Chem. 260, 12273-12279). The hydrophobic carboxyl reagent N,N'-dicyclohexylcarbodiimide (DCCD) inactivates the enzyme with pseudo-first(More)
A field surveying effort was conducted over a one year period to develop an equation that relates flow rate to depth for a flowing spillway (dike) in Lancaster, California. The development of the equation, which is the main objective of this project, is quite valuable, because it helps determine flow rate from simple depth measurements under challenging(More)