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1. We have identified a Ca(2+)-mobilizing receptor that responds to extracellular ATP (0.1-1000 microM) in a salivary epithelial cell line (ST885). The activated receptor also stimulates Ca2+ entry from the extracellular fluid. 2. The receptor shows an agonist selectivity profile consistent with the behaviour of the nucleotide receptor class. In particular,(More)
Atopic eczema is a chronic skin condition affecting between 5% and 20% of children aged up to 11 years, and the numbers are increasing. While eczema is often seen to be a minor problem, research has shown that it can cause considerable disruption to the lives of children and their carers, and can incur significant cost for the family and the healthcare(More)
We have reported previously that cytosolic pH (pHi) in the mouse 2-cell conceptus is controlled by a H+-monocarboxylate cotransporter (MCT) that is sensitive to cinnamates and p-chloromercuriphenylsulfonate. In the present study we have used measurement of pHi with BCECF to characterize the changes in MCT activity during pre-implantation development. We(More)
In this study we used imaging techniques with the fluorescent pH-sensitive dye 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein to investigate the control of cytosolic pH (pHi) in two-cell mouse embryos in nominally HCO3(-)-free conditions. We found that the resting pHi of two-cell embryos (40-50 h after human chorionic gonadotropin) in HCO3(-)-free M2 was(More)
Intracellular pH recovery in Quackenbush Swiss mouse preimplantation embryos following acid loading was investigated under conditions of H+-monocarboxylate cotransporter inactivity. Isoform-sensitive inhibitors of Na+-H+ exchange (NHE) were used to block the Na+-dependent component of the response. A biphasic dose-response curve for HOE-694 and(More)