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Evidence for the existence of an effective interfacial tension between miscible fluids: isobutyric acid-water and 1-butanol-water in a spinning-drop tensiometer.
Effective interfacial tension values measured over time were practically constant, while the interface between the two phases remains sharp as the volume of the drop declines, and the effective interf facial tension was found to be insensitive to changes in temperature and always larger than the equilibrium interfacial pressure.
Chemical cytometry: fluorescence-based single-cell analysis.
It is now possible to detect yoctomole amounts of many analytes in single cells and to employ capillary electrophoresis separation and ultrasensitive laser-induced fluorescence detection.
Evaluation of capillary zone electrophoresis for charge heterogeneity testing of monoclonal antibodies.
Metabolic cytometry. Glycosphingolipid metabolism in single cells.
Electrophoretic profiles were remarkably reproducible, which facilitated identification of components based on the migration time of fluorescently labeled standards, so that trace metabolites can be detected in the presence of abundant components.
Yoctomole analysis of ganglioside metabolism in PC12 cellular homogenates
An ultrasensitive method based on migration of two components was employed andabolite that preserves the fluorescent label can be detected at the yoctomole level, which should allow characterization of this metabolic pathway in single cells.
Instrumentation for medium-throughput two-dimensional capillary electrophoresis with laser-induced fluorescence detection.
A multiplexed system that allows separation of five samples in parallel that produces detection limits of 940 +/- 350 yoctomoles for 3-(2-furoyl)quinoline-2-carboxaldehyde labeled trypsin inhibitor in one-dimensional separation and detection limits degrade by a factor of 3.8 for two-dimensional separations.
Capillary electrophoresis‐mass spectrometry methods for tryptic peptide mapping of therapeutic antibodies
Tryptic peptide mapping is routinely used in the biotech industry to confirm primary sequence, cell line stability, and to analyze posttranslational modifications. Peptide analysis is generally done
CE‐MALDI interface based on inkjet technology
An ink jet printer valve and a nozzle were used to deliver matrix and sample from an electrophoresis capillary onto a MALDI plate and over 70% coverage was obtained for the tryptic digest of α‐lactalbumin.