Claus Pelling

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The structure of polytene chromosomes has been observed by conventional scanning electron microscopy and also with a high resolution ‘in lens’ field emission instrument. Surface imaging with secondary electron emission has characterized condensed chromatin and regions known for their RNA synthetic potential (nucleoli, Balbiani rings and puffs). In DNA-rich(More)
Intra- and interprotamine cross-linking by disulphide bonds are the main factors responsible for the highly compact and stable structure of chromatin in mammalian spermatozoa. Unfixed or methanol fixed smears of human sperm and sperm suspensions from fertile donors and oligospermic patients were subjected to a reductive cleavage of disulphide bonds by using(More)
Cellular autoradiography was used to measure relative rates of chromosomal RNA synthesis and to examine the regulatory phenomenon of X-linked dosage compensation in Drosophila miranda, a species containing two distinct, nonhomologous X chromosomes (X1 and X2). The X1 chromosome was found to be dosage-compensated, since the rate of RNA synthesis along the(More)
Nach Injektionen der Larven des Bastards Chironomus thummi thummi × Chironomus thummi piger mit H3-Thymidin, sowie C14-Thymidin und H3-Thymidin in zeitlichem Abstand, wird der Verlauf der DNS-Synthese in den Speicheldrüsen-Chromosomen autoradiographisch analysiert. Der DNS-Replikationsschritt beginnt gleichzeitig in allen Querscheiben mit einer(More)
The stoichiometry of metachromatic staining of chromatin by toluidine blue was investigated in isolated metaphase chromosomes from L929 cells using X-ray microanalysis. Microspectrophotometric measurements revealed that a hypsochromic shift (from 595 to 570 nm) occurs in toluidine blue stained chromosomes in relation to the staining solution. Under the(More)