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The effect of cyclopiazonic acid, an inhibitor of sarcoplasmic reticulum Ca(2+)-ATPase, was investigated on force generation by the contractile apparatus in Triton-skinned fibres from extensor digitorum longus and soleus. Concentrations of cyclopiazonic acid lower than 10 microM were without effect on Ca(2+)-activated tension in both types of muscles. In(More)
1. Trabeculae isolated from ferret heart and from other mammalian hearts have been mounted in a way that enables the tension generated to be measured while the composition of the bathing fluid is rapidly altered.2. Application of caffeine to these trabeculae initiates a rapid transient contracture and depresses the strength of regularly evoked heart(More)
1. A comparison of the delayed outward current of isolated fibres from rat soleus and iliacus muscle has been made using a double sucrose-gap voltage-clamp method. 2. The fast and slow components of the outward current were separated using time constants of the tail currents. The results indicate that in both iliacus and soleus fibres there is a shift in(More)
1. A double sucrose gap technique has been combined with an optical method of measuring the movement of the preparation and applied to rat ventricular trabeculae.2. Experiments performed in normal McEwen and in depleted sodium solutions with and without tetrodotoxin, manganese or predepolarization suggest that the fast inward current is not as important as(More)
In frog atrial fibres, cyclopiazonic acid as well as thapsigargin, which are inhibitors of sarcoplasmic reticulum Ca(2+)-ATPase, induced a significant increase in the twitch amplitude without detectable changes in its kinetics. The measurements performed on chemically skinned fibres show that cyclopiazonic acid has no effect on the properties of contractile(More)
1. The ionic currents in slow fibres isolated from rat soleus muscle have been studied under voltage-clamp conditions with a double sucrose-gap method and the results are compared to those obtained from fast fibres isolated from the iliacus muscle. 2. The mean value of the resting potential in slow fibres is -70 mV. a value 8 mV more positive that the mean(More)
The inotropic effects of external calcium concentration ([Ca2+]o] and rest periods have been compared in papillary muscles isolated from control (n = 4) and pressure-overloaded right (n = 5) ventricles of adult ferrets. Hypertrophy was induced by pulmonary artery clipping for 30-45 days. Under control conditions (3 mM [Ca2+]o, 0.1 Hz), the isometric twitch(More)
Slow-twitch and fast-twitch muscle fibres have specific contractile properties to respond to specific needs. Since sodium current density is higher in fast-twitch than in slow-twitch fibres, sodium channels contribute to the phenotypic feature of myofibres. Phenotype determination is not irreversible: after periods of rat hindlimb unloading (HU), a model of(More)
Disuse of postural slow-twitch muscles, as it occurs in hypogravity, induces a slow-to-fast myofibre type transition. Nothing is known about the effects of weightlessness on the resting membrane chloride conductance (gCl), which controls sarcolemma excitability and influences fibre type transition during development and adult life. Using the current-clamp(More)
Because of the role of intracellular Na on cardiac contractility and of the depressed isometric contractile response of the hypertrophied myocardium, the effects of pressure overload on the intracellular Na activity (aiNa) have been investigated in papillary muscles isolated from the ferret right ventricle. In animals subjected to pulmonary artery clipped(More)