Clarina Pascual

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OBJECTIVE To compare total plasma antioxidant capacity and selected individual antioxidants in patients with varying degrees of severity of sepsis. DESIGN A prospective, observational, consecutive case study. SETTING A 16-bed intensive care unit (ICU) in a university teaching hospital. INTERVENTIONS None. PATIENTS Forty-six healthy controls, ten ICU(More)
OBJECTIVE To evaluate the effect of intravenous (i.v.) access in children undergoing bilateral myringotomy with pressure-equalizing tube placement. DESIGN One hundred healthy children were enrolled in this randomized controlled study. One group received i.v. access; the other group did not. Anesthesia in both groups was induced through a mask and(More)
Objective: To compare the respiratory burst of neutrophils in sepsis and control patients using lipopolysaccharide (LPS), autologous plasma, and a combination of the two. Design: Prospective, consecutive case study. Setting: A 16-bed intensive care unit (ICU) in a university teaching hospital. Interventions: None. Patients: Plasma was obtained from 23(More)
The use of dried blood spot (DBS) specimens in quantitative alpha1-antitrypsin (alpha1-AT) detection or genetic analysis is limited because protein levels in the samples are low and they contain components that can interfere with polymerase chain reaction amplification. A methodological adaptation was developed to overcome these drawbacks which is discussed(More)
A rapid high-performance liquid chromatographic method was developed using a short silica column (30 mm x 4.6 mm) with an aqueous methanol mobile phase consisting of methanol-water-NH4H2PO4 (94:5.96:0.04) adjusted to a final apparent pH of 5.0 and pumped at a flow-rate of 1 ml/min. Ultraviolet detection was carried out at a wavelength of 280 nm, and serum(More)
Analysis of nondeproteinized samples with an enzymatic method to determine D-lactate indicated interferences. The presence of L-lactate dehydrogenase (LD) and L-lactate in the sample led to underestimation of D-lactate content when a sample blank was processed and overestimation when it was omitted. We proved that this interference is not due to lack of(More)
The reaction between luminol as a chemiluminescence probe and 3-morpholinosydnonimine (SIN-1) as a peroxynitrite donor was evaluated in order to determine the action of several antioxidants. Several well-known antioxidants found in biological fluids or cells modify the light profile of the reaction between SIN-1 and luminol. One main modification was(More)
Girls with a history of premature pubarche, i. e. appearance of pubic hair before 8 years of age, show hyperinsulinism in response to an oral glucose tolerance test. As hyperinsulinaemia has a major role in dyslipaemia, and is considered an independent risk factor for cardiovascular disease, we assessed the patterns of plasma insulin concentration after a(More)
To characterize the molecular basis of the "new" alpha1-antitrypsin (alpha1AT) deficient variant, PI Y barcelona, DNA sequence analysis of the coding exons of the alpha1AT gene was carried out using an amplification DNA technique and direct sequencing. The PI Y barcelona allele differs from the normal M1(Val213) allele sequence by two point substitutions: a(More)
The concentration of gamma interferon (IFN gamma) and adenosine deaminase (ADA) activity were measured in the pleural fluid of 162 patients to compare their diagnostic significance and to establish a possible correlation between both tests. The IFN gamma levels in tuberculous pleural effusions were quite variable, with a mean of 93 U/ml and a median of 48(More)