Claire M. Wolfrom

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The proliferative activity of long-term cultured mammalian cells exhibits traits of a complex dynamic system, with a succession of spontaneous rises and falls in proliferation rate. We analyzed three successive series of proliferation data for the Fao hepatoma cell line in long-term cultures. In the three series the proliferation rate displayed apparently(More)
The growth rate of human skin fibroblasts was evaluated when glucose was replaced by fructose in the culture medium. Four mediums containing respectively 5.5 mmol/l glucose (G1), 27.5 mmol/l glucose (G5), 5.5 mmol/l fructose (F1), and 27.5 mmol/fructose (F5) were used. Skin fibroblasts from fourteen subjects were continuously cultured for 20 days and the(More)
Two types of human fibroblast strains were studied in culture. One was derived from abdomen skin and the other from abdominal muscle aponeurosis. Tissue-specific differences were found between these two cell strains. Skin fibroblasts had faster doubling time, smaller cell volume, and lower glucose consumption when compared to aponeurosis fibroblasts.(More)
After successful ascorbate and manganese treatment of a female patient with prolidase deficiency and iminodipeptiduria, we attempted to explain the mechanism of action of these drugsin vitro, using them preferentially on skin fibroblasts. Sincein vivo, ascorbate and manganese seemed to be responsible for both biochemical and clinical improvement, they were(More)
The activity of Glutamine Synthetase (GS) was measured during the growth of human diploid skin fibro-blasts cultured for three weeks in the presence or absence of either glucose or glutamine or both. In medium free of both glucose and glutamine, a single late peak in GS activity was observed concomitantly with delayed small cell protein increment. In all(More)
The combined effects of carbohydrates and glutamine were investigated in diploid strains of normal human skin fibroblasts cultured for 21 days under eight different culture conditions: hexose-free medium or medium containing D-glucose, D-galactose, or D-fructose, with or without added glutamine. Cell growth, hexose consumption, lactate production,(More)
To evaluate the influence of cell density on the activity of fibroblast prolidase (EC, we determined this activity in sparse and dense cultures. We also investigated, the effects of different concentrations of beta-D (-) fructose and L (+) ascorbate, which both increased cell density at confluency. For a fructose concentration of 25 mM, we(More)
The effect of fructose as a substitute for glucose in cell culture media was investigated in human skin fibroblast and liver cell cultures. Cells were grown for between 2 and 10 days in identical flasks in four different media, containing 5.5, mmol·1−1 and 27.5 mmol·I−1 glucose and fructose, respectively. In the presence of fructose, cell growth was(More)
This study focused on the activation/differentiation of human microglial cells and astrocytes, which is a prerequisite for HIV1 replication. In vitro, IFN gamma induced a differentiation-like morphological change in embryonic microglia and astrocytes, in both primary and in purified culture. This effect was enhanced by TNF alpha which in itself had no(More)
Immortal cells perpetuate the rises and falls of proliferation that are progressively damped in mortal long-term cultured cells. For immortal rat hepatoma Fao cells, similar waves of proliferation occurred about every 3–4 wk. Under the same conditions, embryonic human fibroblasts and transformed but not immortalized embryonic fibroblasts display similarly(More)