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This paper deals with the development of a microfluidic biochip for the exposure of living cells to nanosecond pulsed electric fields (nsPEF). When exposed to ultra short electric pulses (typical duration of 3-10ns), disturbances on the plasma membrane and on the intra cellular components occur, modifying the behavioral response of cells exposed to drugs or(More)
Human adipose mesenchymal stem cells (haMSCs) are multipotent adult stem cells of great interest in regenerative medicine or oncology. They present spontaneous calcium oscillations related to cell cycle progression or differentiation but the correlation between these events is still unclear. Indeed, it is difficult to mimic haMSCs spontaneous calcium(More)
In this paper, we present a novel microfluidic system dedicated to the application of ultra short pulses (i.e. nanopulses) on cells and the visualization of their effects. Cell plasma membranes can be rendered permeable by the use of nanosecond pulsed electric field. In conventional macroscopic electroporation chambers, the typical pulse duration is on the(More)
The biological mechanisms induced by the application of nanosecond pulsed electric fields (nsPEFs: high electrical field amplitude during very short duration) on cells remain partly misunderstood. In this context, there is an increasing need for tools that allow the delivering of such pulses with the possibility to monitor their effects in real-time. Thanks(More)
SdFFF is now commonly used for cell sorting. Nevertheless, as with many other separation methods, SdFFF Hyperlayer elution leads (1) to sample dilution resulting in cell loss which could restrict further use; and (2) to a high output flow rate impacting detector sensitivity and selectivity. In order to limit these problems, we proposed modifications of the(More)
Dielectrophoresis is an efficient technique often used to handle or trap micro-/nano-particles within microfluidic devices. For that purpose, arrays of insulating materials disposed between polarization electrodes are classically used to generate the necessary dielectrophoresis forces. In this paper, a novel method is proposed where metallic micro dots are(More)
Dielectrophoresis (DEP) is broadly used in microfluidic systems for the cell therapies or medical diagnostics [1] because of its capability to handle and sort biological cells [2,3]. In this paper, a new method to trap cells on-chip with high density arraying capabilities is proposed. The principle is based on the use of metallic singularities arrayed(More)
A new biochip for adherent cell trapping using micro patterned parylene-C is presented. We demonstrate the ability of micro patterned parylene-C films to provide an efficient positioning and orientation of cells. It is also possible to fix one cell separate from the others which may be crucial in the development of biodevices for the single-cell analysis.(More)
This paper deals with the development of a biochip dedicated to fusion between two types of cells, in a highly parallelized way. The fusion process is initiated by an electric field pulse applied to cells paired in fluidic traps. The paper will present different strategies for the capture and pairing of cells prior to the electrofusion, combining fluidic(More)
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