Ciro Ferreira Da-Silva

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INTRODUCTION Severe lesions in the facial nerve may have extensive axonal loss and leave isolated stumps that impose technical difficulties for nerve grafting. METHODS We evaluated bone marrow stem cells (BMSC) in a silicone conduit for rat facial nerve regeneration from isolated stumps. Group A utilized empty silicone tubes; in groups B-D, the tube was(More)
The tube repair method was used to study peripheral nerve regeneration in five different inbred mouse strains. The sciatic nerve of male adult mice of the C57BL/6J, DBA/1J, C3H/HeJ, BALB/cJ and A/J strains (N = 3) was cut and both proximal and distal nerve stumps were inserted into a polyethylene tube leaving a 4-mm nerve gap. After 6 weeks the tubes(More)
Tubulization repair technique is a useful model to study peripheral nerve regeneration by offering quantifiable parameters to assess the possible effect of exogenously applied substances on nerve repair. In the present study we demonstrated that locally administered GM1 inside a tubular prosthesis at the time of implantation can significantly improve the(More)
The sciatic nerve of adult mice was transected and the proximal and distal nerve stumps were sutured into a polyethylene tube. The tubes were implanted either empty, or the lumen was filled with pure collagen or a mixture of collagen/nerve growth factor (NGF). Six weeks later, cells in the L3-L5 dorsal root ganglia (DRG) were retrogradely filled with(More)
The tubulization repair technique is a useful model for studying peripheral nerve regeneration since it provides quantifiable parameters for assessing the effects of exogenously applied substances on nerve repair. In this study, we observed that the local administration of hyaluronic acid (HA) in a tubular prosthesis at the time of implantation(More)
The proximal and distal stumps of severed mouse sciatic nerves were inserted into opposite ends of polyethylene tubes. The tubes were implanted either empty or filled with collagen alone or in combination with interleukin-1 (IL1). Six weeks later, neurons in the L3-L5 dorsal root ganglia were back-filled with HRP. The number of HRP reactive sensory neurons(More)
Adult male mice received sciatic nerve transection at the midthigh level and both nerve stumps were sutured into a polyethylene tube (PT) to bridge a nerve gap of 4 mm. The tubes were implanted either empty, or filled with collagen alone or in combination with gangliosides (GM1, GD1a, GD1b and GT1b). Following a survival time of 6 weeks, the PT with the(More)
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