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Since its cloning and commercial availability, applications of green fluorescent protein (GFP) as a reporter gene have become prevalent in many aspects of science. The attributes of GFP could also be applied to the area of heterologous protein production. The work described here represents the first experiments to use GFP as a generic tool to monitor(More)
Potentially damaging reactive oxygen species (ROS) are involved in a number of pathways ranging from signal transduction to apoptosis. Cells have adapted this alteration in redox status into a complex regulatory mechanism. ROS are specifically able to induce the expression of a multitude of genes. We constructed and characterized "oxidative stress probes"(More)
The use of green fluorescent protein (GFP) as a reporter gene has made a broad impact in several areas, especially in studies of protein trafficking, localization, and expression analysis. GFP's many advantages are that it is small, autocatalytic, and does not require fixation, cell disruption, or the addition of cofactors or substrates. Two characteristics(More)
Brazil has one of the highest avifauna diversities in the world, and many species are used by human populations in many different ways. Capturing wild birds for food, or to raise as pets, or for commercial purposes, together with the loss of habitat, have been the principal causes of population reductions among many species. The present work provides new(More)
We present an intensity based sensor designed for on-line monitoring of green fluorescent protein, a revolutionary marker of protein expression. The device consisted of a blue light emitting diode as the excitation source. A band pass excitation filter cut off light longer than 490 nm. The light was directed into a bifurcated optical fiber bundle with the(More)
An all-solid-state green fluorescent protein (GFP) sensor for GFP measurement was developed. It is immune to interference from ambient light and works with standard flow-through cuvettes. The sensor is practically insensitive to the scattered excitation light encountered in microbial suspensions. It has a range of 0.0002-1 g/L (7.4 x 10(-9) - 3.7 x 10(-5)(More)
Green fluorescent protein fusions were constructed with several oxidative stress promoters from Escherichia coli. These promoters were chosen for their induction by reactive oxygen species (ROS) such as superoxide, hydrogen peroxide, and hydroxyl radicals. When exposed to various free radical insults, the cells fluoresced with great specificity based on the(More)
The Atlantic Forest of northeast Brazil hosts a unique biota which is among the most threatened in the Neotropics. Near-total conversion of forest habitat to sugar cane monocultures has left the region’s endemic forest-dependent avifauna marooned in a few highly-fragmented and degraded forest remnants. Here we summarise the current status of 16 globally(More)
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