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We examined the genetic diversity, population structure and gene flow in a dominant mangrove tree (Rhizophora stylosa) at its northern biogeographical limit in Sakishima islands of the Japanese archipelago. Simple sequence repeat (SSR) markers from chloroplast (cpSSR) and nuclear DNA were used to analyze 16 populations recovered from various river basins(More)
Ectomycorrhizal fungal (EMF) propagules play an important role in seedling establishment following disturbance. However, little is known about how the EMF propagule community changes with forest development. In this study, EMF propagules were examined using seedling bioassays in rhizosphere soils collected from a recently closed Pb–Zn tailing (Taolin Pb–Zn(More)
Two colour forms of the two-spotted spider mite (Tetranychus urticae Koch) coexist in China: a red (carmine) form, which is considered to be native and a green form which is considered to be invasive. The population genetic diversity and population genetic structure of this organism were unclear in China, and there is a controversy over whether they(More)
We analyzed nuclear and chloroplast microsatellite makers to assess genetic diversity and examine genetic structure of two mangrove tree species, Bruguiera gymnorrhiza and Kandelia obovata recovered from nine major river basins of Iriomote Island of the Ryukyu Archipelago, Japan. The average number of alleles per nuclear locus per population was 2.6 in B.(More)
To advance our understanding of ectomycorrhizal fungal communities in mining areas, the diversity and composition of ectomycorrhizal fungi associated with Masson pine (Pinus massoniana Lamb.) and soil chemistry were investigated in Taolin lead–zinc (Pb–Zn) mine tailings (TLT), two fragmented forest patches in a Huayuan Pb–Zn mineland (HY1 and HY2), and a(More)
Anemonefishes are popular model species for research on population connectivity via larval dispersal, and understanding this connectivity is beneficial when designing marine protected areas. We developed 15 microsatellite markers for two anemonefishes, Amphiprion frenatus and Amphiprion perideraion. Of these 15 markers, 10 worked well for both species,(More)
Pieris japonica is a poisonous tree species that is rarely eaten by herbivorous animals, which could allow it to expand its distribution and change ecosystems. Using a next-generation sequencer, 18 microsatellite markers were isolated from P. japonica and characterized. The number of alleles at each locus ranged from 2 to 11. The observed and expected(More)
Sour jujube or wild jujube (Ziziphus acidojujuba), considered as the ancestor of cultivated jujube, is an economically and ecologically important species. However, little is known about the population genetics of this species across China. In this study, we detected genetic variation among 874 individuals from 31 natural populations covering the most(More)
It is still controversial whether ectomycorrhizal (ECM) mycelia filter out toxic metals in nutrient absorption of host trees. In this study, pine (Pinus densiflora) seedlings colonized by Cu-sensitive and Cu-tolerant ECM species were exposed to a wide spectrum of soil Cu concentrations to investigate functions of ECM fungi under Cu stress. The(More)
To understand the reproduction of the pioneer ectomycorrhizal fungi Laccaria amethystina and Laccaria laccata in a volcanic desert on Mount Fuji, Japan, the in situ genet dynamics of sporocarps were analysed. Sporocarps of the two Laccaria species were sampled at fine and large scales for 3 and 2 consecutive years, respectively, and were genotyped using(More)