Chung Soo Chung

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As a step towards understanding the molecular mechanism of adipogenesis in pigs, preadipocytes purified from the back fat of 1 day-old female piglets were used for in vitro culture. Normalized cDNA libraries were constructed with 1.6×107 and 1.1×107 independent clones from preadipocyte and mature adipocyte mRNAs, respectively. Polymerase chain reaction(More)
The technique of Rodbell (J. Biol. Chem. 239: 375) was modified considerably in order to isolate swine adipocytes without rupturing large cells. Cell size and diameter distributions were the same for adipocytes fixed with OsO4 following isolation with collagenase and adipoctes liberated from OsO4-fixed adipose tissue slices. Lipogenic rates were greater for(More)
The early neonatal development of boars is characterized by significant testicular production of androgens and estrogens, including an anabolic steroid hormone, 19-nortestosterone. The present study was conducted to determine the expression and presence of steroidogenic and steroid hormone metabolism-related enzymes in the testes of neonatal and 4-month-old(More)
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