Chun-qing Cai

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The present study was designed to investigate the electrophysiological characteristics of rat conduit pulmonary artery smooth muscle cells (PASMCs) and the response to acute hypoxia. PASMCs of the 1st to 2nd order branches in the conduit pulmonary arteries were obtained by enzymatic isolation. The PASMCs were divided into acute hypoxia preconditioned group(More)
OBJECTIVE To study the effects of doxofylline on calcium-activated potassium (K(Ca)) channels of human peripheral blood eosinophils in asthma. METHODS Peripheral blood eosinophils from patients with asthma were isolated and divided equally into two groups, a control group and a doxofylline incubated group. The data were recorded using cell-attached(More)
To investigate the role of ion channels in the coupling responses of neutrophils to extracellular stimulus, it is necessary to study the membrane ion channel activities using patch-clamp technique. However, little has been known about the ion channel activities in neutrophils due to the difficulties in forming giga-seal with pipettes because of small(More)
AIM To validate the abundance of Interleukin 8 receptor beta (IL-8Rbeta) mRNA in single human neutrophil. METHODS Human neutrophils were isolated and purified from volunteers, total RNA was extracted and a regular RT-PCR aiming at IL-8Rbeta mRNA was performed to ascertain its expression profile in human neutrophils and optimize the reaction conditions for(More)
OBJECTIVE To determine the optimal method for separating neutrophils for studying neutrophil polarization. METHODS Human neutrophil was separated from healthy human peripheral blood by Percoll density gradient centrifugation and Dextran sedimentation. The cell polarization, purity and activity of the neutrophils were determined, and F-actin polymerization(More)
A modified protocol for quick and economic treatment of cultured human umbilical vein endothelial cells has been established, which result in high viability of the cells to allow better performance in patch-clamp studies. The electrophysiological properties of Ca (2+)-activated K(+) (KCa) channel of the cultured cells were investigated with a cell-attached(More)
AIM AND METHODS To observe the effect of temperature on burst opening of voltage-dependent K+ channels(Kv) in hypothalamic neurons by cell-attached mode of patch-clamp technique. RESULTS With temperature raising, the number of burst opening increased, so did its average burst duration. B1 and B2 raised from 1.5 ms and 6.6 ms at 32 degrees C to 8.1 ms and(More)
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