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In preclinical studies in rats we evaluated biodistribution and therapeutic effects of different somatostatin analogs, [(111)In-DTPA]octreotide, [(90)Y-DOTA,Tyr(3)]octreotide and [(177)Lu-DOTA,Tyr(3)]octreotate, currently also being applied in clinical radionuclide therapy studies. [Tyr(3)]octreotide and [Tyr(3)]octreotate, chelated with DTPA or DOTA, both(More)
The satellite cell is responsible for growth and repair of postnatal skeletal muscle. We investigated the expression of the myogenic regulatory gene (MRG) family in these cells in the stages from quiescence to fusion. Using polymerase chain reaction amplification of reverse-transcribed RNA (RT-PCR) isolated from adult rat satellite cells, we demonstrated a(More)
Using a reverse transcription/polymerase chain reaction method, the temporal pattern of expression of the myogenic regulatory genes (myf5, myogenin, MRF4, myo D) was quantitated in developing mouse muscle (whole embryo: 6.5 to 12.5 days postcoitum (dpc); front limb buds: 9.5 to 12.5 dpc; hind limb buds: 11.5 to 14.5 dpc) and related to expression of(More)
myf5 is one of a family of four myogenic determination genes that control skeletal muscle differentiation. To study the role of myf5 in vivo, we generated transgenic mice harboring the bovine homolog, bmyf, under control of the murine sarcoma virus promoter. Ectopic expression of the full-length bmyf transgene was detected in brain and heart tissue samples(More)
We have shown by receptor-binding analyses that the beta-2 adrenergic receptor is present on rat ROS 17/2.8 osteoblast-like cells. This was confirmed by PCR amplification of cDNA copied from the mRNA. The beta-1 adrenoreceptor subtype was absent and its mRNA was not detectable, even at the level of sensitivity afforded by PCR analysis. The beta-adrenergic(More)
We report the novel synthesis of polymerase chain reaction (PCR)-derived single-stranded DNA (ssDNA) probes and their subsequent application in in situ hybridizations. Serial transverse sections of an 11.5-day postcoitum mouse embryo were hybridized to a 33P-ssDNA, 33P-RNA, or 35S-RNA probe corresponding to the same 181-bp sequence in the myogenin cDNA.(More)
In this paper, we describe the wiki markup language WikiCreole, how it was developed, and related work. Creole does not replace existing markup, but instead enables wiki users to transfer content seamlessly across wikis, and for novice users to contribute more easily. In proposing a subset of markup elements that is as non-controversial as possible, this(More)
Through the use of two internal controls, we have developed an improved method of quantitating ribonuclease protection assay (RPA) results. A truncated sense RNA fragment and an antisense RNA fragment for the gene of interest were transcribed from PCR fragments containing T7 bacterial promoters. An 18S ribosomal RNA fragment was also used. When radiolabeled(More)
In becoming wiki editors, non-technical users face many obstacles, one of the largest being that every wiki has its own unique syntax. A common wiki markup would facilitate not only learning and teaching wikis, but also developing advanced wiki editors and tools. The wiki markup standard should be intuitive and unlikely to interfere with existing text.(More)
The following report outlines the use of the polymerase chain reaction (PCR) in combination with in vitro transcription to generate single-stranded radiolabelled RNA probes useful for nuclease protection and in situ hybridization experiments. Specific DNA fragments with bacteriophage promoter (T3 and/or T7) sequences at the 5' or 3' end are generated by(More)