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PURPOSE The goal of this study was to evaluate the effect of oxygen deprivation on rhodopsin regeneration in the excised mouse eye. METHODS A new preparation for studying rhodopsin regeneration with a superfused excised albino mouse eye was developed. The preparation exhibits multiple regenerations after moderate bleaches (15%-20%) and is sensitive to the… (More)
A short review of the threading approach to protein structure prediction, including presentation of some open statistical problems. Also discussed is one of the likely sources of the current limited success, that being the form of the pairwise potentials used in most threading approaches.
The beta subunit of the heterotrimeric G proteins that transduce signals across the plasma membrane is made up of an amino-terminal alpha-helical segment followed by seven repeating units called WD (Trp-Asp) repeats that occur in about 140 different proteins. The seven WD repeats in Gbeta, the only WD repeat protein whose crystal structure is known, form… (More)
PURPOSE To evaluate the effect of diabetes on rhodopsin regeneration in the excised mouse eye. METHODS A superfused excised mouse eye preparation that exhibits rhodopsin regeneration after moderate bleaches and that is responsive to the composition of the perfusate was used. Diabetes was induced in albino mice (BALB/c) with the diabetogenic agent… (More)
To study the process of rhodopsin regeneration a superfused excised whole eye preparation of the albino mouse was developed. With this preparation, complete regeneration could be observed after each of the first two illuminations (bleaching 15-20%), and incomplete regeneration after a third illumination. Regeneration was minimal at extracellular glucose… (More)