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CEL I, isolated from celery, is the first eukaryotic nuclease known that cleaves DNA with high specificity at sites of base-substitution mismatch and DNA distortion. The enzyme requires Mg(2+) and Zn(2+) for activity, with a pH optimum at neutral pH. We have purified CEL I 33 000-fold to apparent homogeneity. A key improvement is the use of(More)
The purposes and functions of semitransparent eye protective curtains are analyzed. Based upon this analysis, functional requirements are developed, and design requirements are specified for optimum curtains to be used with open arc welding and arc cutting processes. Such curtains also protect against other high intensity broad-spectral-band sources such as(More)
Mixed urinary incontinence (MUI) can be a challenging condition to manage. We describe the protocol design and rationale for the Effects of Surgical Treatment Enhanced with Exercise for Mixed Urinary Incontinence (ESTEEM) trial, designed to compare a combined conservative and surgical treatment approach versus surgery alone for improving patient-centered(More)
A synoptic of programmatic and technical lessons learned in the development of aerothermodynamic data bases for the X-33 and X-34 programs is presented in general terms and from the perspective of the NASA Langley Research Center Aerothermodynamics Branch. The format used is that of the Òaerothermodynamic chain,Ó the links of which are personnel,(More)
The large JPL solar simulation systems are unique in their ability to furnish well collimated light over large areas with good uniformity and high efficiency/intensity. These systems furnish simulated solar irradiance to 10-ft (3-m) and 25-ft (7.6-m) space simulation chambers. The discussion is divided into two parts. In the first part (Sec. II) the(More)
During January–April 2007, Project Recovery, a federally funded crisis counseling program implemented by Mississippi’s Department of Mental Health, piloted a new model of Specialized Crisis Counseling Services (SCCS) on the Mississippi Gulf Coast. In this team-based approach, a masters-level counselor trained in a variety of intervention techniques and a(More)
An automated DNA synthesis protocol (FC3) has been optimized to decrease the cost of chemical consumption by two- to threefold. Anion-exchange chromatography at pH 12.5 in a Mono Q (Pharmacia) column was used to analyze the oligonucleotides we synthesized. From these analyses, we formulated the FC3 program and a procedure by which a DNA synthesizer designer(More)