In April 2009, a new influenza A (H1N1 2009) virus emerged that rapidly spread around the world. While current variants of this virus have caused widespread disease, particularly in vulnerable groups, there remains the possibility that future variants may cause increased virulence, drug resistance or vaccine escape. Early detection of these virus variants… (More)
An oseltamivir-resistant influenza A pandemic (H1N1) 2009 virus evolved and emerged from zero to 52% of detectable virus within 48 hours of a patient's exposure to oseltamivir. Phylogenetic analysis and data gathered by pyrosequencing and cloning directly on clinical samples suggest that the mutant emerged de novo.
DNA microarrays used as 'genomic sensors' have great potential in clinical diagnostics. Biases inherent in random PCR-amplification, cross-hybridization effects, and inadequate microarray analysis, however, limit detection sensitivity and specificity. Here, we have studied the relationships between viral amplification efficiency, hybridization signal, and… (More)
BACKGROUND Pathogen detection using DNA microarrays has the potential to become a fast and comprehensive diagnostics tool. However, since pathogen detection chips currently utilize random primers rather than specific primers for the RT-PCR step, bias inherent in random PCR amplification becomes a serious problem that causes large inaccuracies in… (More)
BACKGROUND Transmission of hepatitis B virus (HBV) is rare within healthcare settings in developed countries. The aim of the article is to outline the process of identification and management of transmission of acute hepatitis B in a renal inpatient ward. METHODS The case was identified through routine reporting to public health specialists, and… (More)
Technical Appendix Figure. Timeline of a multidrug-resistant tuberculosis (MDR TB) outbreak associated with a local area network (LAN) gaming cafe in Singapore. During February–December 2012, MDR TB infection was confirmed in 5 persons (case-patients A–E) who patronized the cafe. A minimum spanning tree based on single nucleotide polymorphism (SNP) analysis… (More)
Office Mon 2-3pm, Wed 1-2pm Mon 9-10am, Fr 4-5pm Tue 10-11am, Wed 3-4pm hours * or by appointment * The TA office hours will be held in Jack's Lounge (in the Baskin Engineering building). Course description (from the registrar): Introduction to statistical methods/reasoning, including descriptive methods, data-gathering (experimental design and sample… (More)