Christopher H. George

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The assembly of gap junction intercellular communication channels was studied by analysis of the molecular basis of the dysfunction of connexin 32 mutations associated with the X-linked form of Charcot-Marie-Tooth disease in which peripheral nervous transmission is impaired. A cell-free translation system showed that six recombinant connexin 32 mutated(More)
Chimeric proteins comprising connexins 26, 32, and 43 and aequorin, a chemiluminescent calcium indicator, were made by fusing the amino terminus of aequorin to the carboxyl terminus of connexins. The retention of function by the chimeric partners was investigated. Connexin 32-aequorin and connexin 43-aequorin retained chemiluminescent activity whereas that(More)
The normal contractile, electrical, and energetic function of the heart depends on the synchronization of biological oscillators and signal integrators that make up cellular signaling networks. In this review we interpret experimental data from molecular, cellular, and transgenic models of cardiac signaling behavior in the context of established concepts in(More)
Although antigen-loaded dendritic cells (DC) are being investigated as antitumor vaccines, which DC differentiation state is most effective is not clear. Three DC functions that may be critical for immunization potential are expression of CD80/86, cytokine production following CD40 engagement, and migration to chemokine receptor 7-binding chemokines. We(More)
The emergence of human stem cell-derived cardiomyocyte (hSCCM)-based assays in the cardiovascular (CV) drug discovery sphere requires the development of improved systems for interrogating the rich information that these cell models have the potential to yield. We developed a new analytical framework termed SALVO (synchronization, amplitude, length, and(More)
Calcium is the primary signalling component of excitation-contraction coupling, the process linking electrical excitability of cardiac muscle cells to coordinated contraction of the heart. Understanding $${\text{Ca}}^{2+}$$ Ca 2 +  handling processes at the cellular level and the role of intercellular communication in the emergence of multicellular(More)
BACKGROUND AND PURPOSE Flecainide is a use-dependent blocker of cardiac Na(+) channels. Mechanistic analysis of this block showed that the cationic form of flecainide enters the cytosolic vestibule of the open Na(+) channel. Flecainide is also effective in the treatment of catecholaminergic polymorphic ventricular tachycardia but, in this condition, its(More)
We propose to demonstrate the Open Language Interface for Voice Exploitation (OLIVE) speech-processing system, which SRI International developed under the DARPA Robust Automatic Transcription of Speech (RATS) program. The technology underlying OLIVE was designed to achieve robustness to high levels of noise and distortion for speech activity detection(More)
Questioning flecainide's mechanism of action in the treatment of catecholaminergic polymorphic ventricular tachycardia Flecainide attenuates cardiac Ca 2+ cycling abnormalities in malignant catecholamine-triggered arrhythmias but its mechanism of action remains highly contentious. We read with interest the study of Yang et al. (2016) that used in silico(More)