Christopher Blackwell

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1. The 120-fold purification of ethanolamine ammonia-lyase from Escherichia coli extracts, to apparent homogeneity, is described. Ethanolamine, dithiothreitol, glycerol and KCl protected the apoenzyme from inactivation. 2. At the optimum pH7.5, K(m) values for ethanolamine and coenzyme B(12) were 44mum and 0.42mum respectively. The K(m) for ethanolamine was(More)
Induction of ethanolamine ammonia-lyase formation in Escherichia coli required both the ethanolamine and vitamin B12, and was gratuitous during growth on glycerol. Ethanolamine analogues inhibited enzyme activity and inhibited growth with ethanolamine as the the nitrogen source, but did not act as inducers. Enzyme formation was more rapid when ethanolamine(More)
1. Kinetic studies of ethanolamine ammonia-lyase formation by Escherichia coli suggested that coenzyme B12 (5'-deoxyadenosylcobalamin), with ethanolamine, is a co-inducer. 2. Enzymic and immunological tests failed to show the formation of complementary enzyme components induced separately by ethanolamine and cobalamin respectively. 3. Although specific for(More)
In this paper, a novel approach is introduced for classifying curves into proper families, according to their similarity. First, a mathematical quantity we call plane curvature is introduced and a number of propositions are stated and proved. Proper similarity measures of two curves are introduced and a subsequent statistical analysis is applied. First, the(More)
  • C Blackwell
  • 1998
In some applications, the slow response of measurement systems can present significant control problems or cause difficulties in interpreting data sequences, even though they are quite accurate in indicating the value of an unchanging measured parameter. Happily, instrument systems often have characteristics which make it possible to apply certain advanced(More)
OBJECTIVE The objective of this study was to compare the long-term outcome of patients with metastatic melanoma vaccinated with 6MHP to that of a group of unvaccinated historical controls. BACKGROUND A multipeptide vaccine (6MHP), designed to induce helper T cells against melanocytic and cancer-testis antigens, has been shown to induce specific(More)