Christopher Barney

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IL-2 potentiates both growth and cytotoxic function of T lymphocytes and NK cells. Resting peripheral blood NK cells can respond directly to rIL-2, without requirement for accessory cells or cofactors, and enhanced cytotoxicity can be measured within a few hours after exposure to this lymphokine. In this study, we describe an activation antigen, Leu-23,(More)
The vasopressin-activated calcium-mobilizing (VACM-1) protein is a unique arginine vasopressin (AVP) receptor which shares sequence homology with the cullins, genes involved in the regulation of cell cycle transitions. Unlike either cullins or AVP receptors, however, VACM-1 is expressed exclusively in the vascular endothelial cells and in the renal(More)
The relative contribution of cellular and extracellular water deficits to the genesis of thirst due to thermal dehydration was studied in male Sprague-Dawley rats following exposure to a 40 degrees C environment for 4 h. Intragastric (I.G.) and intravenous (I.V.) preloads of water reduced the elevated plasma sodium and plasma osmolality of thermally(More)
Vasopressin-activated calcium-mobilizing (VACM-1), a cul-5 gene, is localized on chromosome 11q22-23 close to the gene for Ataxia Telangiectasia in a region associated with a loss of heterozygosity in breast cancer tumor samples. To examine the biological role of VACM-1, we studied the effect of VACM-1 expression on cellular growth and gene expression in(More)
The aim of the present study was to characterize the sympathetic nerve responses to hyperthermia in chloralose-anesthetized rats. Discharges were recorded from the renal, lumbar, and splanchnic sympathetic nerves. Mean arterial pressure, heart rate, and sympathetic nerve discharge (SND) were recorded continuously during progressive increases in core body(More)
Spontaneously hypertensive (SH) rats have been shown to have elevated colonic temperatures when compared with normotensive Wistar-Kyoto (WKY) rats. In the present study, core temperatures of SH and WKY rats were compared using radiotelemetry temperature sensors implanted in the abdominal cavity. At an ambient temperature (Ta) of 25 degrees C, SH and WKY(More)
Temporal variation in spontaneous water intake in rats is well established but little is known about temporal variation in water intake following dehydration. In the present study different male Sprague-Dawley strain rats were exposed without water for 3 h to either a 25 degrees C or a 40 degrees C environment every 4 h for 20 h. The rats were then allowed(More)
VACM-1, a cul5 gene product, when overexpressed in vitro, has an antiproliferative effect. In vivo, VACM-1/cul5 is present in tissues involved in the regulation of water balance. Neither proteins targeted for VACM-1/cul5-specific degradation nor factors that may regulate its expression in those tissues have been studied. To identify genes that may be(More)
Male Sprague-Dawley rats were utilized to study the effects of the opioid receptor antagonists, naloxone and naltrexone, on thirst induced by thermal dehydration. In an initial experiment, the depressant effect of naloxone (1.0 mg/kg, IP) on the water intake of rats deprived of water for 24 h was confirmed. In subsequent experiments, rats were thermally(More)
Spontaneously hypertensive (SH) rats and normotensive Wistar-Kyoto (WKY) rats were exposed to either 25 or 37.5 degrees C for 3.5 h, and their thermal and water balance responses were compared. After exposure, either a blood sample was obtained or the rats were allowed to rehydrate for 4 h. SH rats had both higher core temperatures and evaporative water(More)