Christopher A. Cullis

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A single-copy 5.7 kilobase (kb) DNA fragment, termed Linum Insertion Sequence 1 (LIS-1), has been identified and characterized. This is one of the DNA changes associated with the environmentally induced heritable changes resulting in stable lines termed genotrophs in flax (Linum usitatissimum). The insertion sequence and its insertion site have been cloned(More)
Some flax varieties respond to nutrient stress by modifying their genome and these modifications can be inherited through many generations. Also associated with these genomic changes are heritable phenotypic variations. The flax variety Stormont Cirrus (Pl) when grown under three different nutrient conditions can either remain inducible (under the control(More)
Agrobacterium-mediated plant transformation via floral-dip is a widely used technique in the field of plant transformation and has been reported to be successful for many plant species. However, flax (Linum usitatissimum) transformation by floral-dip has not been reported. The goal of this protocol is to establish that Agrobacterium and the floral-dip(More)
The opinions expressed in this report are those solely of the colloquium participants. are equally amenable to use in large or small scale farming operations and compatible with other agricultural practices and technologies. Concerns associated with the use of Bt include potential for harm to non-target organisms, development of resistance in populations of(More)
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