Christophe Jacob

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A growing number of yeast and mammalian plasma membrane proteins are reported to be modified with K63-linked ubiquitin (Ub) chains. However, the relative importance of this modification versus monoubiquitylation in endocytosis, Golgi to endosome traffic, and sorting into the multivesicular body (MVB) pathway remains unclear. In this study, we show that(More)
Stress hormones, adrenaline (epinephrine) and noradrenaline (norepinephrine), are responsible for many adaptations both at rest and during exercise. Since their discovery, thousands of studies have focused on these two catecholamines and their importance in many adaptive processes to different stressors such as exercise, hypoglycaemia, hypoxia and heat(More)
One significant problem in monitoring a culture's evolution is to assess change in cell viability. We have demonstrated that LDH release could be a good indicator of cellular damage of many cell lines, especially during shear stress or sonication. Moreover, we have found a significant correlation between the number of dead cells, determined by Trypan Blue(More)
This review focuses on recent evidence that identifies potential extracellular and cellular mechanisms that may be involved in the tolerance of ectomycorrhizal fungi to excess metals in their environment. It appears likely that mechanisms described in the nonmycorrhizal fungal species are used in the ectomycorrhizal fungi as well. These include mechanisms(More)
* Metallothioneins are small polypeptides involved in metal tolerance of many eukaryotes. Here we characterized the Pimt1 gene, coding for a metallothionein from the ectomycorrhizal fungus Paxillus involutus. * Expression of Pimt1 in P. involutus under metal stress conditions was measured by northern blot and RT-PCR analyses. The full-length cDNA was used(More)
The molecular mechanisms underlying the response of ectomycorrhizal fungi to heavy metals in general and cadmium in particular remain poorly understood. We screened 2040 arrayed cDNAs of the ectomycorrhizal fungus Paxillus involutus to identify cadmium-responsive genes by using differential hybridization. Forty nine (2.4%) of the 2040 cDNAs were(More)
BACKGROUND The aim of the present study was to investigate recent overweight and obesity prevalence rates for Lebanese adolescents, and to examine differences in physical activity, screen time (sum of time spent in front of TV, computer, and videogames), and health-related quality of life (HRQOL) for the first time among normal, overweight, and obese(More)
PURPOSE The aim of this study was to compare the whole body (WB) bone mineral content (BMC) and bone mineral density (BMD) in obese, overweight, and normal-weighted adolescent sedentary girls. METHODS This study included 17 obese, 27 overweight, and 21 normal-weighted adolescent (aged, 12-20) sedentary (practicing less than 2 hours of physical(More)
The purpose of this study was to compare, during a 30s intermittent exercise (IE), the effects of exercise intensity on time spent above 90% $${\dot{\hbox{V}}\hbox{O}}_{{{\rm 2max}}} (t90\,{\dot{\hbox{V}}\hbox{O}}_{{{\rm 2max}}})$$ and time spent above 95% $${\dot{\hbox{V}}\hbox{O}}_{{{\rm 2max}}} (t95\,{\dot{\hbox{V}}\hbox{O}}_{{{\rm 2max}}})$$ in young(More)
Being overweight is associated with increased bone mineral content, bone mineral density (BMD), and bone strength in adults. However, the effect of being overweight on bone strength during adolescence is poorly understood. The aim of this study was to compare femoral neck geometry in overweight and normal weight adolescent girls. This study included 22(More)