Christophe Breuil

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The quotient of the upper half plane by Γ1(N), acting by fractional linear transformations, is the complex manifold associated to an affine algebraic curve Y1(N)/C. This curve has a natural model Y1(N)/Q, which for N > 3 is a fine moduli scheme for elliptic curves with a point of exact order N . We will let X1(N) denote the smooth projective curve which(More)
Identification of key enzymes of sapstain fungi which cause wood discoloration is necessary for targeted inhibition strategies. Therefore proteinases involved in the nitrogen pathway have been characterized. The sap-staining fungus Ophiostoma piceae strain 387N produced proteolytic enzymes when grown on wood and protein-supplemented media. Proteolytic(More)
A proteinase secreted by the sapstaining fungus Ophiostoma piceae is thought to be necessary for the primary retrieval of nitrogen from wood proteins. By using mass spectrometry (MS) techniques, we have established the cleavage specificity of this subtilisin-like serine proteinase. This work demonstrated the potential of MS in determining cleavage(More)
The extracellular serine proteinase secreted by Ophiostoma piceae was degraded by autoproteolysis under certain conditions. At elevated temperatures the mature protein of 33 kDa was rapidly degraded without any accumulation of protein breakdown products. Glycerol, calcium ions and ammonium sulfate raised the heat stability of the enzyme, increasing its(More)
Scytalone dehydratase is involved in the production of fungal dihydroxynaphthalene (DHN) melanin. We have isolated and characterized OSD1, a gene encoding scytalone dehydratase from the sap-staining fungus Ophiostoma floccosum by PCR-based cloning. Sequence analysis suggests that the OSD1 gene encodes a protein of 216 amino acids with a molecular weight of(More)
Two different DNA sequences amplified by PCR from Ophiostoma floccosum show high homology to reductase genes of the fungal melanin biosynthetic pathway and suggest that two reductases may participate in this pathway in O. floccosum. Previously, one reductase gene, THN1 had been isolated from O. floccosum and proved to be involved in melanin biosynthesis.(More)
The Canadian Ophiostoma Genome Project, which was initiated in 2001, is a collaborative effort between research teams in four different universities. Its general objective is to conduct a large-scale identification and analysis of genes controlling important aspects of the life cycle of Ophiostomatoid fungi. To this end, several expressed sequence tag (EST)(More)