Christoph Metzner

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Lipid rafts have been proposed as sites for the assembly of a number of viruses and are considered to play a major role in pseudotyping events. As a consequence, host glycosylphosphatidylinositol (GPI) anchored proteins commonly associated with lipid rafts can be found being incorporated into viral lipid envelopes with beneficial consequences for viral(More)
In this study, we describe a versatile, flexible, and quick method to label different families of enveloped viruses with glycosylphosphatidylinositol-modified green fluorescent protein, termed fluorescence molecular painting (FMP). As an example for a potential application, we investigated virus attachment by means of flow cytometry to determine if viral(More)
We describe for the first time the association of glycosylphosphatidylinositol (GPI) -anchored proteins with retroviral and lentiviral particles, similar to a process well established for cells, termed "painting." The aim of the study was to assess the feasibility of modification of retroviral vectors by exogenous addition of recombinant protein, removing(More)
Gene delivery vectors based on retroviral or lentiviral particles are considered powerful tools for biomedicine and biotechnology applications. Such vectors require modification at the genomic level in the form of rearrangements to allow introduction of desired genes and regulatory elements (genotypic modification) as well as engineering of the physical(More)
Providing information about single virus particles has for a long time been mainly the domain of electron microscopy. More recently, technologies have been developed-or adapted from other fields, such as nanotechnology-to allow for the real-time quantification of physical virion particles, while supplying additional information such as particle diameter(More)
Mouse mammary tumour virus (MMTV) encodes a viral superantigen (Sag) and a negative acting factor (Naf) which share parts of their coding sequence. Using 2-dimensional gel electrophoresis (2D-DIGE), we could show that at least 10 different cellular proteins were differentially expressed in Naf positive cells. Also, luciferase reporter expression was(More)
The majority of tinnitus cases are related to cochlear dysfunction, leading to altered peripheral input to the central auditory system [1]. These alterations are believed to increase the basic level of neural activity during off-conditions of sound and to diminish the increase in neural activity when sound is presented [2]. As a compensatory means the(More)
Context integration is an ubiquitious principle in corti-cal processing underlying many perceptual and cognitive functions. Several neuropsychiatric disorders have been associated with an impairment of integration of context information, particularly schizophrenia [5]. One way to investigate the mechanisms underlying context processing and its impairments,(More)
Context integration is an ubiquitious principle in corti-cal processing underlying many perceptual and cognitive functions. Several neuropsychiatric disorders have been associated with an impairment of integration of context information, particularly schizophrenia [5]. One way to investigate the mechanisms underlying context processing and its impairments,(More)
Over the last decades an enormous amount of studies have been conducted searching for biomarkers of psychiatric disorders on the molecular level, aiming at improving diagnostics and therapy thereof. However, the gap between the genetic/molecular scale and the behavioural scale is huge and genes with substantial contributions have not been found for most(More)