Christine Smyth

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In an earlier study exploring the potential of gene transfer to repair myocardial conduction defects, we observed that myotubes, generated by forced expression of MyoD, exhibit reduced excitability when also modified to express connexin43 (Cx43). We hypothesized that this effect was caused by gap junction-mediated coupling between myotubes and the(More)
Dendritic cells (DC) are among the most potent antigen-presenting cells known and play an important role in the initiation of antigen-specific T-lymphocyte responses. Several recent studies have demonstrated that DC expressing vector-encoded tumor-associated antigens can induce protective and therapeutic immunity in murine cancer models. In the current(More)
Mutations in the gene encoding the common gamma chain (gammac) of interleukin receptors 2, 4, 7, 9, 15 and 21 result in X-linked severe combined immunodeficiency (SCID-X1). Classically, this disease is characterised by an absence of T and NK cells, and near normal numbers of functionally deficient B cells (B(+), T(-), NK(-) phenotype). Atypical phenotypes(More)
Because little is known about elder abuse, it is difficult to propose strategies for primary prevention or to select the most efficacious strategies to deal with abuse. Intervening is also complicated by personal and professional values that often have their origin in one's own family. Elder abuse is a complex challenge to health professionals, making(More)
Cluster ensembles seek a consensus across many individual partitions and the resulting solution is usually stable. Cluster ensembles are well suited to the analysis of DNA microarrays, where the tremendous size of the dataset can thwart the discovery of stable groups. Post processing cluster ensembles, where each individual partition is weighted according(More)
OBJECTIVE To report the outcome of gene therapy in an infant with X-linked severe combined immunodeficiency (SCID-X1), which typically causes a lack of T and natural killer (NK) cells. DESIGN AND SETTING Ex-vivo culture and gene transfer procedures were performed at The Children's Hospital at Westmead, Sydney, NSW, in March 2002. Follow-up to March 2005(More)
Cell ploidy and proliferative activity may be useful to clinicians in treating children with solid tumors. Frozen specimens from children with malignant solid tumors were tested at the time of diagnosis for ploidy and proliferative activity. The flow cytometric DNA histograms were examined to assess the sensitivity of the tumor cell preparation and staining(More)
In the process of developing a cancer immunotherapy strategy, we have identified and characterized a novel intracellular reservoir of CD86 protein in peripheral blood monocytes. This observation emerged from studies aimed at using retrovirus vectors to genetically modify tumor cells to express the costimulatory proteins CD80 and CD86. Retrovirus-mediated(More)