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The purpose of this in vitro study was to determine whether the substitution of 0.12% chlorhexidine gluconate for sterile water as a mixing agent would enhance the antimicrobial activity of tooth-colored ProRoot mineral trioxide aggregate (MTA) against Actinomyces odontolyticus (ATCC17982), Fusobacterium nucleatum (ATCC2586), Streptococcus sanguis(More)
AIM To test the hypothesis that the mechanical efficacy of irrigation in reducing bacteria in the root canal is dependent on depth of placement of the irrigation needle. METHODOLOGY The root canals of 30 permanent cuspids were instrumented to apical size 60 using a crown-down technique. A suspension of the bioluminescent reporter strain Pseudomonas(More)
This study tested the hypothesis that the mechanical efficacy of irrigation in reducing intracanal bacteria is dependent on canal preparation size. Root canals of 30 extracted permanent cuspids were prepared consecutively to sizes 36, 60, and 77 at working length (WL) using ProFile 0.04 taper Series-29 files. Smear layer was removed and teeth autoclaved(More)
A 4-year longitudinal study of the oral prevalence of enteric gram-negative rods and yeasts in 116 Chinese primary school children in Hong Kong was conducted. The oral prevalence of enteric gram-negative rods for each consecutive year was 25.3%, 37.0%, 24.0% and 25.8% respectively, with a weighted mean of 27.9%. Enterobacteriaceae, which comprised 57% of(More)
AIM The hypotheses tested in this study were that: (i) Enterococcus faecalis can survive long-term entombment in root filled teeth without additional nutrients, (ii) initial cell density influences the survival of E. faecalis in instrumented root canals and (iii) gelatinase-production capacity influences the survival of E. faecalis in root canals. (More)
There are no quantitative data on the mechanical efficacy of irrigation in the removal of bacteria from curved canals. This study quantitatively analyzed the effects of root canal curvature and preparation size on the mechanical efficacy of irrigation using 33 mandibular single-rooted bicuspids allocated to groups according to root canal curvatures, group 1(More)
This study investigated the prevalence, phenotype and genotype of oral enterococci. Enterococci were detected in oral rinse samples from 11% of 100 patients receiving endodontic treatment and 1% of 100 dental students with no history of endodontic treatment (P=0.0027). All enterococcal isolates were identified as Enterococcus faecalis. Viable counts ranged(More)
Interactions between Enterococcus faecalis and other species found in root canal infections might be important for the development and persistence of periapical disease. The aim of this study was to investigate the coaggregation interactions between E. faecalis clinical isolates and species previously shown to survive and induce apical periodontitis in(More)
Enterococcus faecalis strain OG1RF and its (p)ppGpp-deficient ΔrelA, ΔrelQ, and ΔrelA ΔrelQ mutants were grown in biofilms and evaluated for growth profiles, biofilm morphology, cell viability, and proteolytic activity. E. faecalis lacking (p)ppGpp had a diminished capacity to sustain biofilm formation over an extended period of time and expressed abundant(More)