Christine L. Tetzlaff

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Peripheral blood mononuclear cells from cattle experimentally infected with Babesia bovis were examined for parasite-specific cell-mediated immune responses. Unfractionated merozoites and soluble and membrane fractions derived from merozoites were all antigenic for immune cattle, although the membrane fraction was the most stimulatory. Cattle responded to(More)
Previous studies have demonstrated the serologic and T-cell immunogenicity for cattle of a recombinant form of the apical complex-associated 77-kDa merozite protein of Babesia bovis, designated Bb-1. The present study characterizes the immunogenic epitopes of the Bb-1 protein. A series of recombinant truncated fusion proteins spanning the majority of the(More)
Alveolar macrophages were isolated from lung lavage fluids taken from BCG-vaccinated and nonvaccinated guinea pigs 2 and 4 wk after challenge with virulent Mycobacterium tuberculosis H37Rv by the respiratory route. Animals had been maintained on either purified, protein-adequate (30%) or protein-deficient (10%) isocaloric diets for 6 wk prior to respiratory(More)
Specific-pathogen-free Hartley guinea pigs were maintained on isocaloric-purified diets either adequate (30%) or moderately deficient (10%) in protein. Half of each diet group was vaccinated with viable Mycobacterium bovis BCG. Six weeks later, all animals were challenged by the respiratory route with virulent Mycobacterium tuberculosis H37Rv. At intervals(More)
Specific pathogen-free, Hartley guinea pigs were vaccinated with viable Bacille Calmette-Guerin (BCG) and given isocaloric diets identical in every nutrient except protein (control = 30%; low protein = 10%). A non-vaccinated group was maintained on the control diet. Five weeks later, all animals were infected with an aerosol containing virulent M.(More)
A major portion of a Babesia bovis-specific gene encoding a 77-kDa merozoite protein (Bb-1) produced during natural infection in cattle and in microaerophilous culture was subcloned into the pGEX1N expression vector. Recombinant Bb-1 protein fused to glutathione S-transferase (Bb-1-GST) was used to examine cellular immune responses in B. bovis-immune(More)
Babesia microti genomic DNA was purified from parasitized murine erythrocytes, digested with mung bean nuclease and used to construct an expression library in lambda gt11. Polyspecific antisera from mice infected with virulent B. microti organisms (ATCC30221) were used to screen the genomic library for genes encoding major immunogens. High titer antisera(More)
The influence of acute dietary protein restriction on the development ofBabesia microti infection in the mouse model was investigated. Female mice consuming a diet either devoid of protein or adequate with respect to protein were infected withB. microti-parasitized erythrocytes and sacrificed 7 days later. Absence of dietary protein resulted in a delay in(More)
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