Christine G. Bowden

Learn More
The hydrophobic protein cerato-ulmin (CU), produced by Ophiostoma ulmi, has been implicated in the pathogencity of this fungus on elm. Primers were designed based on the nucleotide sequence deduced from the published CU amino-acid sequence, and a DNA fragment of the cu gene was amplified using the polymerase chain reaction. The amplified cu fragment was(More)
The button mushroom, Agaricus bisporus, is a commercially important cultivated filamentous fungus. During the last decade, the button mushroom industry has depended mainly on two strains (or derivatives of these two strains). Using one of these highly successful strains (strain U1) we examined the phenomenon of strain instability, specifically, the(More)
The COL1 gene was isolated from Ophiostoma novo-ulmi using the techniques of insertional mutagenesis and plasmid rescue. Sequence analyses suggest that the COL1 gene encodes a unique protein of 826 amino acids with consensus-type RNA-binding domains, most similar to a putative protein from Schizosaccharomyces pombe which resembles the C-terminus of the(More)
Cerato-ulmin (CU), a hydrophobin produced by Ophiostoma novo-ulmi, has been implicated in the pathogenicity of this fungus on elm. We have generated a CU- mutant by transformation-mediated gene disruption of a highly virulent (aggressive) strain of O. novo-ulmi. The inability of the mutant to synthesize CU was confirmed by transcript analysis as well as(More)
Little genetic information exists comparing aggressive and non-aggressive isolates of the causal agent of Dutch elm disease,Ophiostoma ulmi. Two genetic elements were compared between the subgroups. The ceratoulmincu gene product has been associated with disease symptoms. Nucleotide-sequence analysis ofcu and the internal transcribed spacer (ITS) region of(More)
  • 1