Christina Gruber

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Trans-splicing is a powerful approach to reprogram the genome. It can be used to replace 5', 3' or internal exons. The latter approach has been characterized by low efficiency, as the requirements to promote internal trans-splicing are largely uncharacterized. The trans-splicing process is induced by engineered 'RNA trans-splicing molecules' (RTMs), which(More)
Spliceosome-mediated RNA trans-splicing has become an emergent tool for the repair of mutated pre-mRNAs in the treatment of genetic diseases. RNA trans-splicing molecules (RTMs) are designed to induce a specific trans-splicing reaction via a binding domain for a respective target pre-mRNA region. A previously established reporter-based screening system(More)
BACKGROUND Recessive dystrophic epidermolysis bullosa (RDEB) is a hereditary skin disorder characterized by mechanical fragility of the skin, resulting in blistering and chronic wounds. The causative mutations lie in the COL7A1 gene. Patients suffering from RDEB have a high risk to develop aggressive, rapidly metastasizing squamous cell carcinomas (SCCs).(More)
INTRODUCTION Airway management is an important component of cardiopulmonary resuscitation (CPR). Recent guidelines recommend keeping any interruptions of chest compressions as short as possible and not lasting more than 10 seconds. Endotracheal intubation seems to be the ideal method for establishing a secure airway by experienced providers, but emergency(More)
The aging process of skin has been investigated recently with respect to mitochondrial function and oxidative stress. We have here observed striking phenotypic and clinical similarity between skin aging and recessive dystrophic Epidermolysis bullosa (RDEB), which is caused by recessive mutations in the gene coding for collagen VII,COL7A1. Ultrastructural(More)
Theory-based predictions on asymmetries in animal contests have seldom been tested in situations where payoff asymmetries exist among contestants otherwise clearly matched for physical fighting capacity (i.e., resource holding potential, RHP). We examined the effects of food availability and predation risk on contest outcomes and the maintenance of(More)
Spliceosome-mediated RNA trans-splicing (SMaRT) is a molecular tool that facilitates genetic reprogramming on the RNA level (Wally et al., 2012). SMaRT exploits the cells own splicing machinery to recom-bine two RNA molecules: the endogenous RNA target and the RNA trans-splicing molecule (RTM). The end product is a chimeric RNA wherein part of the message(More)
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