Christie Ramos Andrade

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Herpetomonas samuelpessoai was submitted to hypo- and hyper-osmotic shocks in buffer and culture medium. Cell volume was evaluated with an electronic particle counter and by the transmission of a parasite suspension measured at 540 nm with a spectrophotometer. Cell volume changes after osmotic shock were transient, parasites being able to completely restore(More)
Two-hundred and sixty-five human sera from Brazil were tested in a direct agglutination assay using trypsinated Leishmania donovani donovani antigen. The assay proved to be of value for the diagnosis of American visceral leishmaniasis in that it was discriminative toward Chagas' disease.
The need for a reliable method for the immunological diagnosis of kala-azar is imperative. Leishmania donovani donovani and L. donovani chagasi culture promastigotes were compared as antigens in a direct agglutination test (DAT) for the diagnosis of visceral leishmaniasis in Brazil. Both antigens were successfully employed for the DAT, showing 100%(More)
The redox properties of the hydroethanol extract (EE) and its ethyl acetate (EAF) and hydromethanol (HMF) fractions obtained from Abarema cochliacarpos (Gomes) Barneby & Grimes stem bark were evaluated. EAF had the highest total phenol content (848.62 ± 78.18 mg g⁻¹), while EE showed the highest content of catechin (71.2 µg g⁻¹). EE, EAF and HMF exhibited(More)
L. donovani antigens were analyzed by a direct agglutination test (DAT), by ELISA using intact promastigotes and by the SDS-PAGE immunoperoxidase technique (SGIP). Sera of Chagas' disease patients cross-reacted in the ELISA and SGIP but not in the DAT. Trypsin treatment of the parasites removed concanavalin A-binding sites but not epitopes for antibodies(More)
An enzyme-linked lectin assay (ELLA) based on the ELISA assay, using intact formalin-fixed promastigotes to coat poly-L-lysine-treated microtiter plates is described. The assay was used to study the lectin receptors of Leishmania donovani chagasi, L. donovani donovani and L. mexicana amazonensis. ConA, RCA, WGA, and PNA receptors were found in the three(More)
Diseases presenting with splenomegaly, fever and pancytopenia require intensive differential diagnostic considerations. These diseases include lymphoproliferative and autoimmune diseases, but also chronic infections like mansonian schistosomiasis or visceral leishmaniasis (kala-azar). Diagnosis for the latter is usually performed by testing for the presence(More)
Corticotropin-releasing factor (CRF) is a peptide that is released from the hypothalamus into widespread areas of the brain. Evidence has suggested that CRF is involved as a neuromodulator outside of the hypothalamic-pituitary-adrenal axis, playing an important role in fear, anxiety, depression and pain modulation. Our previous report demonstrated that CRF(More)
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