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Tuberculosis due to Mycobacterium spp. continues to represent a major threat to human and animal health, prompting the search for effective vaccines. We have previously reported a sequential prime and boost homologous vaccination regime, using live avirulent M. bovis bacille Calmette-Guerin (BCG) strain 1173P2, that can provide significant protection to red(More)
SETTING Three commercial deer herds, each containing more than 500 deer, experienced outbreaks of tuberculosis (TB) ranging from a 6% prevalence to disease levels >50%. Intensive diagnostic testing was carried out over short time intervals after the initial diagnosis of TB. OBJECTIVE To eradicate M. bovis infection from herds of farmed red deer (Cervus(More)
A blood test for tuberculosis in deer was developed as an ancillary test to clarify the status of skin test-positive deer, with non-specific sensitisation following exposure to saprophytic mycobacteria. The blood test incorporates the measurement of the relative humoral and cellular immunological responses to Mycobacterium bovis and M. avium antigens to(More)
This study was designed to develop a customized enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of Johne's disease (JD) in farmed deer. Two antigens were selected on the basis of their superior diagnostic readouts: denatured purified protein derivative (PPDj) and undenatured protoplasmic antigen (PpAg). ELISA development was based on the(More)
BCG has been used widely as a vaccine to prevent tuberculosis (TB) for 80 years, yet there is still considerable controversy about its efficacy. Many experimental variables have obscured the true efficacy of BCG. The absence of appropriate animal models for the study of protective efficacy and the lack of in vitro correlates of protective immunity have(More)
Mycobacterium bovis infection produces tubercular lymphadenitis in the head lymphatics of cattle and deer, in addition to pulmonary disease. A low-dose intra-tonsilar infection model that establishes tuberculosis (Tb) lymphadenitis in cattle and deer is characterised in this study. Intra-tonsilar infection of red deer (500 cfus of M. bovis) was monitored(More)
Long-term neuroleptic administration produces a behavioral supersensitivity to dopamine agonists. Tyrosine hydroxylase immunoreactive synapses in the striatum are closely associated with putative glutamate-mediated synapses, on dendrites and dendritic spines of the same neuronal population. The purpose of the present study was to determine whether chronic(More)
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